Commissariat a L'energie Atomique

Bertin Et Cie

Hla-g Technologies

Jean Dausset

Edgardo Delfino Carosella

Nathalie Rouas-Freiss

Pascale Paul

Marek Kirszenbaum

Philippe Moreau

Daniel Cohen

Philippe Millasseau

Yves Le Gall

Isabelle Le Gall

The invention concerns a method for selecting tumours expressing HLA-G, sensitive to an anticancer treatment, which inhibits or prevents the HLA-G activity of said tumours and the uses thereof. Said method enable to establish either the HLA-G, transcription profile of a solid tumour or the HLA-G expression profile of a solid tumour. The method for establishing the HLA-G transcription profile consists in: (i) drawing a tumoral sample; (ii) extracting the mRNA; (iii) reverse transcription (RT) of said RNA: (iv) successive or simultaneous amplification of the cDNA's obtained in (iii) in the presence of primers specific to each HLA-G isoform and analysing the resulting amplification products, by electrophoresis and/or specific hybridisation and (v) establishing said sample HLA-G transcription profile. The method for establishing the HLA-G expression profile consists in: (i) drawing a tumoral sample; (ii) optionally marking said sample cells; (iii) carrying out a lysis of the cells; (iv) contacting said cells which have been subjected to lysis with different antibodies directed against the class I HLA-G antigens, to form, optionally HLA-G isform/antibodies complexes; and (v) establishing said sample HLA-G expression profile by detecting the complexes formed in step (iv).

Method for selecting tumours expressing HLA-G, sensitive to anticancer treatment and uses

The invention concerns the use of a eukaryotic cell coding for at least an HLA-G isoform, to obtain an immunomodulating medicine for inhibiting the activity of killer cells, in particular NK cells and/or for inhibiting the primary allogenic response, in parthologies or situations in which the killer cells are activated or for preparing a medicine to raise the inhibiting function of the isoform(s) expressed by said cells with respect to killer cells, in particular NK cells, in pathologies where these killer cells are inhibited by the molecule of the major histocompatibility complex of I HLA-G classes. The invention also concerns eukaryotic cells expressing at their surface at least an HLA-G isoform and their applications. The invention further concerns transgenic animals specifically expressing at least an HLA-G isoform.

Eukaryotic cells expressing at their surface at least an HLA-G isoform and their applications

A method and apparatus for apportioning a primary volume of fluid into a plurality of secondary volumes having a predefined mutual relationship, by means of a chamber for receiving the primary volume of fluid having a bottom including fluid evacuation orifices (6) disposed in a common horizontal plane and connected to evacuation ducts (8) provided with cocks (19), enabling the beginning of fluid flow in the ducts (8) to be synchronized, with the end of said flow being determined by the free surface (26) of the fluid being fractioned by the evacuation orifices (6).

Method and apparatus for apportioning a primary volume of fluid into a

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Paris, France

Jean Dausset