Institut National De La Sante Et De La Recherche Medicale

Association Pour Le Development De La Recherche En Genetique Moleculaire (aderegem)

Jean-Claude Nicolas

Pierre H Chambon

Hinrich Gronemeyer

Daniel Metzger

Beatrice Terouanne

Bernard Descomps

Sylvie Roux

Andre Crastes De Paulet

Jacques Bertrand Brocard

A DNA fragment coding for a modified nuclear glucocorticoid receptor, particularly one mutated in the region coding for the ligand binding domain, so that receptor activity is more strongly inducible by a synthetic glucocorticoid ligand than by a natural glucocorticoid ligand, is disclosed. A recombination system inducible in mammals by means of a fusion protein produced between a recombinase and the binding domain of the ligand derived from the modified glucocorticoid receptor of which the activity is more strongly inducible by synthetic glucocorticoids than by natural glucocorticoids, is also disclosed.

Modified nuclear glucocorticoid receptor, fusion protein, and DNA fragments coding for said receptor and said fusion protein

The invention relates to a method for the selective separation of a given protein substance having an affinity for a ligand from other substances some of them having the same affinity for the ligand but also having a high molecular weight, higher than that of said protein substance, said substances being in solution. The method consists of filtering said substances on a gel which excludes said high molecular weight substances, said gel being coupled with a ligand for which said protein substance has affinity. The method may be applied to enzymatic and especially to immunoenzymatic assays.

Method for the separation of a protein substance from a solution

Growing community of inventors

Montpellier, France

Jean-Claude Nicolas