Institut Pasteur

Centre National De La Recherche Scientifique

Universite Paris 7

Institute Pasteur

Universite Paris Vii

Jean-Claude Manuguerra

Philippe Despres

Sylvie Paulous

Jessica Vanhomwegen

Frédéric Tangy

Pierre Charneau

Chantal Combredet

Nicolas Escriou

Sylvie Van Der Werf

Jean-François Delagneau

Monique Martin

Bernadette Crescenzo-Chaigne

Benoît Callendret

Saliha Azebi

Jean-Michel Betton

Valérie Lorin

Sylvie Gerbaud

Ana Maria Burguiere

Frederik Kunst

Frederick Kunst

Frederik Institut Pasteur Kunst

Frederik Inst Pasteur Bureau Des Brevets Et Inventions Kunst

The present invention provides an immunoassay leading to the rapid and simultaneous detection of antibodies to a wide range of infectious pathogens in biological fluids of infected patients. This immunoassay involves the covalent and oriented coupling of fusion proteins comprising an AGT enzyme and a viral antigen on an identifiable solid support (e.g. fluorescent microspheres). The thus obtained antigen-coupled microspheres show enhanced capture of specific antibodies as compared to antigen-coupled microspheres produced by standard amine coupling procedures.

Multiplex immuno screening assay

The present invention provides kits and assay methods for the early detection of pathogens, precise identification of the etiologic agent, and improved disease surveillance. More specifically, the present invention discloses an immunoassay leading to the rapid and simultaneous detection of antibodies to a wide range of infectious pathogens in biological fluids of infected patients. This immunoassay involves the covalent and oriented coupling of fusion proteins comprising an AGT enzyme and a viral antigen on an identifiable solid support (e.g. fluorescent microspheres), said support being previously coated with an AGT substrate. This coupling is mediated by the irreversible reaction of the AGT enzyme on its substrate. The thus obtained antigen-coupled microspheres show enhanced capture of specific antibodies as compared to antigen-coupled microspheres produced by standard amine coupling procedures. The methods of the invention possess the ability to multiplex, minimize the amount of biological sample, and have enhanced sensitivity and specificity toward target antibodies as compared with classical ELISA or Radio-Immunoprecipitation assays.

The invention relates to a novel strain of severe acute respiratory syndrome (SARS)-associated coronavirus, resulting from a sample collected in Hanoi (Vietnam), reference number 031589, nucleic acid molecules originating from the genome of same, proteins and peptides coded by said nucleic acid molecules and, more specifically, protein N and the applications thereof, for example, as diagnostic reagents and/or as a vaccine.

Strain of SARS-associated coronavirus and applications thereof

Growing community of inventors

Paris, France

Jean-Claude Manuguerra