Monsanto Technology LLC

Jean C Goley

Gregory R Heck

Ty T Vaughn

Tichafa R I Munyikwa

Joanne J Fillatti

Toni Alols Voelker

James Kevin Roberts

Scott C Johnson

Tichafa RI Munyiakawa

This invention relates to methods of controlling gene expression or gene suppression in eukaryotic cells. One aspect of this invention includes modifying the degree of silencing of a target gene by use of a modified suppression element. Another aspect includes providing a eukaryotic cell having a desired phenotype resulting from transcription in the eukaryotic cell of a modified suppression element. Also provided are transgenic eukaryotic cells, transgenic plant cells, plants, and seeds containing modified suppression elements, and useful derivatives of such transgenic plant cells, plants, or seeds, such as food or feed products.

Modified gene silencing

The invention provides methods for selecting nucleotide sequences that yield dsRNA-mediated gene suppression in a target organism and enable their uptake by the target organism. The invention further provides expression constructs that confer stabilized expression of such sequences in a transgenic host cell, and methods for their use. Also provided are organisms, cells and tissues prepared by a method of the invention.

Selecting and stabilizing dsRNA constructs

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Saint Charles, MO, United States of America

Jean C Goley