Dow Agrosciences LLC

Sangamo Therapeutics, Inc.

Agrigenetics, Inc.

Corteva Agriscience LLC

Jayakumar Pon Samuel

Steven Robert Webb

Frank Burroughs

Narasimha Chary Samboju

Gaofeng Lin

Kerrm Y Yau

Frank G Burroughs

W Michael Ainley

Terry R Wright

Donald J Merlo

Justin Michael Lira

Terence Anthony Walsh

Joseph F Petolino

Lakshmi Sastry-Dent

Jeffrey C Miller

Lei Zhang

Dmitry Y Guschin

Zehui Cao

Mark W Zettler

Liu Shen

Cristie Min Dewes

Suraj K Dixit

Fyodor Urnov

Paul R Schmitzer

Gregory J Cost

Dayakar Reddy Pareddy

William M Ainley

Robbi J Garrison

Toby Cicak

Sivarama Reddy Chennareddy

Rodrigo Sarria-Milan

Jeffrey R Beringer

William Michael Ainley

Liu Y Shen

Cristie M Dewes

The subject invention provides novel plants that are not only resistant to 2,4-D, but also to pyridyloxyacetate herbicides. Heretofore, there was no expectation or suggestion that a plant with both of these advantageous properties could be produced by the introduction of a single gene. The subject invention also includes plants that produce one or more enzymes of the subject invention 'stacked' together with one or more other herbicide resistance genes. The subject invention enables novel combinations of herbicides to be used in new ways. Furthermore, the subject invention provides novel methods of preventing the development of, and controlling, strains of weeds that are resistant to one or more herbicides such as glyphosate. The preferred enzyme and gene for use according to the subject invention are referred to herein as AAD-12 (AryloxyAlkanoate Dioxygenase). This highly novel discovery is the basis of significant herbicide tolerant crop trait and selectable marker opportunities.

Herbicide resistance genes

Methods and compositions for gene disruption, gene editing or gene stacking within a FAD2 loci by cleaving, in a site directed manner, a location in a FAD2 gene in a soybean cell, to generate a break in the FAD2 gene and then optionally integrating into the break a nucleic acid molecule of interest is disclosed.

FAD2 performance loci and corresponding target site specific binding proteins capable of inducing targeted breaks

Fad2 performance loci and corresponding target site specific binding proteins capable of inducing targeted breaks

The present disclosure relates in part to a method for identifying a soybean germline transformant from a population of soybean transformants by incorporating a selection agent within rooting medium used in tissue culture during the soybean transformation process. The soybean germline transformants are selected from a population of soybean transformants which are comprised of a combination of non-germline and germline soybean transformants. The soybean non-germline transformants are identified and eliminated early in the transformation process. The soybean germline transformants are identified and selected for culturing into mature soybean plants. The method is readily applicable for screening and obtaining a soybean germline transformant at an early stage in the soybean transformation process.

Soybean transformation method

Disclosed herein are methods and compositions for homology-independent targeted insertion of donor molecules into the genome of a cell.

Methods and compositions for nuclease-mediated targeted integration of transgenes

The present disclosure provides a system and methods for detecting and identifying plant events that contain donor sequences inserted precisely into a targeted genomic loci, and plants and plant cells comprising such targeted genomic loci. The method comprises the steps of amplifying a genomic DNA with a first round of PCR to produce an amplicon from donor sequences inserted in the reverse orientation, wherein the production of the amplicon indicates the presence of the site specific integration event.

Rapid assay for identifying transformants having donor insertion

The present disclosure provides methods for detecting and identifying plant events that contain donor sequences inserted precisely into targeted genomic loci, and plants and plant cells comprising such targeted genomic loci. The method comprises the steps of amplifying a genomic DNA with a first round of PCR to produce a first amplicon from donor sequences inserted in the reverse orientation, amplifying the first amplicon with a second round of PCR and detecting the presence of the second amplicon, wherein the production of the first and second amplicon indicates the presence of the site specific integration event.

Rapid assay for identifying transformants having targeted donor insertion

Provided are methods for introducing a sequence-specific nuclease into a plant cell comprising a cell wall. Methods are provided for genetically or otherwise modifying plants and for treating or preventing disease in plant cells comprising a cell wall.

Nanoparticle mediated delivery of sequence specific nucleases

Provided are methods for introducing a molecule of interest into a plant cell comprising a cell wall. Methods are provided for genetically or otherwise modifying plants and for treating or preventing disease in plant cells comprising a cell wall.

Methods for transferring molecular substances into plant cells

Provided are methods for introducing a molecule of interest into a plant cell having a cell wall by using dendrimers, and optionally one or more Cell Penetrating Peptides (CPPs). Methods are provided for genetically or otherwise modifying plants and for treating or preventing disease in plant cells comprising a cell wall.

Use of dendrimer nanotechnology for delivery of biomolecules into plant cells

Methods for introducing a linear nucleic acid molecule of interest into a cell comprising a cell wall include use of nanoparticles coated with polyethylene glycol. In some embodiments, the cell comprising a cell wall is a plant cell. Methods include genetically or otherwise modifying plants and for treating or preventing disease in plant cells comprising a cell wall. Transgenic plants include a nucleic acid molecule of interest produced by regeneration of whole plants from plant cells transformed with linear nucleic acid molecules.

Linear DNA molecule delivery using PEGylated quantum dots for stable transformation in plants

Growing community of inventors

Carmel, IN, United States of America

Jayakumar Pon Samuel