University of Missouri

Other

Jay J Thelen

Matthew Salie

Michael G Muszynski

Vincent J H Sewalt

Douglas D Randall

Jan A Miernyk

Severin E Stevenson

Yajin Ye

A method of altering fatty acid and/or triacylglycerol production in plants and/or algae is provided. The method includes altering activity levels of alpha-carboxyltransferase (α-CT), a catalytic subunit of acetyl-CoA carboxylase (ACCase) by modulating an expression of at least one carboxyltransferase interaction (CTI) gene encoding at least one CTI protein.

Methods of altering seed weight and seed oil content by manipulating alpha-carboxyl-transferase (a-CT) activity via carboxyl-transferase interaction (CTI) protein expression

The present invention provides a method and means to change fatty acid and ultimately triacylglycerol production in plants and algae. Methods of the invention comprise the step of altering the activity levels of the committed step for de novo fatty acid biosynthesis, acetyl-CoA carboxylases (ACCase). More specifically, methods of the invention directly enhance the activity of ACCase by down-regulating the biotin/lipoyl attachment domain containing (BADC) genes through biotechnology or selective breeding approaches.

Increasing plant oil content by altering a negative regulator of acetyl-CoA carboxylase

The present invention provides a method and means to change fatty acid and ultimately triacylglycerol production in plants and algae. Methods of the invention comprise the step of altering the activity levels of the committed step for de novo fatty acid biosynthesis, acetyl-CoA carboxylases (ACCase). More specifically, methods of the invention directly enhance the activity of ACCase by overexpression of &#x3b1;-CT or a catalytic portion thereof.

Increasing plant oil content by improving activity of acetyl-CoA carboxylase

Increasing plant oil content by altering a negative regulator of acetyl-coa carboxylase

The invention provides a high-throughput method for quantitating plant seed proteins, e.g. seed allergens. Such method involves obtaining a protein sample from the seed; analyzing the sample using mass spectrometry with a set of pre-designed internal standard peptides that are specific to the plant allergens; and monitoring frequencies and intensities of resulting spectra to obtain relative and absolute allergen contents in the seed. The invention also provides a system for high-throughput profiling of plant seed allergens. Such system comprises a set of pre-designed internal standard peptides that are specific to the plant seed allergens and one or more mass spectrometers.

High-throughput quantitation of crop seed proteins

The invention provides methods and compositions relating to altering carbohydrate metabolism and/or composition of plants. The invention provides isolated nucleic acids and their encoded proteins, expression cassettes, host cells, transgenic plants, and antibody compositions.

Growing community of inventors

Columbia, MO, United States of America

Jay J Thelen