Abengoa Bioenergia Nuevas Tecnologias, S.a.

Abengoa Bioenergia Nuevas Technologias, S.a.

Javier Rocha Martín

Bruno Díez García

Noelia Valbuena Crespo

Antonio Javier Moreno Pérez

Dolores Pérez Gómez

Sandra Gavaldá Martín

Laura Sánchez Zamorano

Consolación Álvarez Núñez

Francisco Reyes Sosa

Ana Isabel Platero Gómez

Laura Viñas De La Cruz

Laura Ledesma García

Juan Luis Ramos Martín

Lucía Martín Pérez

María De Los Ángeles Bermúdez Alcántara

Ricardo Arjona Antolín

María De Los Angeles Bermúdez Alcántara

Lucia Martín Pérez

Laura Benítez Casanova

Macarena López Morales

The invention relates to a host cell, preferably acell, which presents a lower expression and/or secretion of non-contributory cellulolytic enzymes, preferably where the non-contributory cellulolytic enzyme is endoglucanase 6 comprising SEQ ID NO: 2, thereby promoting the presence of contributory cellulolytic enzymes in the enzymatic cocktail synthesised by said host cell. The invention also relates to the use of said host cells and the enzymatic cocktails synthesised by said host cells for the production of fermentable sugars of biomass and a method for producing bioproducts, preferably bioethanol, comprising the use of said host cell or the composition according to the invention.

host cell having improved cellulolytic activity and enzymatic compounds produced with same

The invention refers to methods and compositions for stabilizing and increasing the activity of enzymatic mixtures comprising GH61 (PMO or polysaccharide monooxigenase) polypeptides used for the degradation of cellulosic material during the saccharification step of biofuel production processes. This improvement is achieved by the addition of a nickel cation to said enzymatic mixtures before and/or during the saccharification step. Thus, the invention provides compositions comprising PMOs, cellulolytic enzymes and a nickel cation, as well as methods for preparing said compositions and methods for producing fermentable sugars and bioproducts, preferably bioethanol, from cellulosic biomass in which said compositions are used.

Cellulolytic compositions comprising monooxygenase polysaccharide enzymes with improved activity

The present invention relates to variants of cellobiohydrolase, preferably Cbh1, which have greater cellobiohydrolase activity. The invention also relates to a genetic construct, a host cell and to an enzyme composition comprising said variants. The invention further relates to a procedure for producing fermentable sugar and a procedure for producing a bioproduct, such as bioethanol, from cellulose material with the cellobiohydrolase variants, the host cell or the enzyme composition comprising said variants.

Variants of cellobiohydrolase 1

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Seville, Spain

Javier Rocha Martín