Caliper Technologies Corporation

Caliper Life Sciences, Inc.

University of California

Javier Anibal Farinas

H Garrett Wada

Andrea W Chow

Stephane Mouradian

J Wallace Parce

Robert S Dubrow

Anne R Kopf-Sill

Theo Teofanov Nikiforov

Josh Molho

Benjamin N Wang

Irina G Kazakova

Bahram Fathollahi

Jude A Dunne

Marja Liisa Alajoki

Ming Wu

Yevgeny Yurkovetsky

Hayley Wu

Raphaele Gerber

Roger Dettloff

Coleen Hacker

Esther Huang

Thi Ngoc Vy Trinh

The invention provides methods and apparatuses that allow a protein sample to undergo reduction, alkylation, and digestion in a continuous flow process carried out within a microfluidic device. Methods and apparatuses in accordance with the invention can be employed as part of an automated proteomics analysis carried out in an integrated proteomics system.

Method and apparatus for performing peptide digestion on a microfluidic device

A microfluidic device with a filter includes a substrate; a flowpath including a well formed in the substrate in fluid communication with a channel formed in the substrate; and a filter disposed across the flowpath and associated with the channel.

Microfluidic device with a filter

The present invention provides methods and reagents for targeting probes to selected cellular locations, through the expression of specific binding partners to that probe within the cell. In one embodiment, the probes may comprise spectroscopic probe that can be used in a method for localizing a specific binding partner within a cell, and for creating assays for post-translational activities. The invention allows the monitoring of the location of such intracellular specific binding partners over time and in response to stimuli, such as test chemicals. The spectroscopic probes can be used for screening a test chemical for activity. The present invention also includes cells and transgenic organisms comprising the intracellular specific binding partner, wherein the specific binding partner can bind with the spectroscopic probe/ligand conjugate.

Methods and reagents for targeting organic compounds to selected cellular locations

Transmembrane potential measurement methods using cationic dyes, and anionic dyes are provided. Compositions of the cationic and anionic dyes and microfluidic systems which include the dyes and membranes are provided in conjunction with processing elements for transmembrane potential measurements.

Use of Nernstein voltage sensitive dyes in measuring transmembrane voltage

Use of nernstein voltage sensitive dyes in measuring transmembrane voltage

Methods and systems for particle focusing to increase assay throughput and sensitivity in microscale systems are provided. The invention includes methods for providing substantially uniform flow velocity to flowing particles in microfluidic devices. Methods of sorting members of particle populations, such as cells and various subcellular components are also provided. Integrated systems in which particles are focused and/or sorted are additionally included.

Focusing of microparticles in microfluidic systems

Compositions for monitoring transmembrane potential across cellular membranes. The compositions typically comprise a cell having a plasma membrane that comprises a first leaflet and a second leaflet, the membrane comprising first and second membrane associated components which, when placed adjacent each other either produce or quench a fluorescent signal, wherein. The first membrane associated component translocates from a first leaflet of the membrane to a second leaflet of the membrane in response to an electrical potential gradient across the membrane, the first membrane associated component being selected from a non-fluorescent cationic fluorescence quencher a non-fluorescent anionic fluorescence quencher and a cationic fluorophore.

Optical detection of transmembrane potential changes

Intracellular binding reactions, and particularly DNA/DNA binding protein reactions are detected in situ, using intracellular fluorescence polarization detection. The methods comprise providing a biological cell having at least a first component of a binding reaction disposed therein. The cell is contacted with a second component of the binding reaction whereby the second component is internalized within the biological cell. At least one of the first and second components has a fluorescent label. The amount of binding between the first and second components within the cell is determined by measuring a level of polarized and/or depolarized fluorescence emitted from within the biological cell.

Methods and systems for monitoring intracellular binding reactions

Growing community of inventors

San Carlos, CA, United States of America

Javier Anibal Farinas