The Charles Stark Draper Laboratory, Inc.

Sample6 Technologies, Inc.

Institute for Environmental Health, Inc.

Jason Wyatt Holder

Connor McBrine

Parker Dow

Sarah Gruszka

Georgiana Kourepenos

Michael Koeris

Daniel Robert Brownell

Miles Rogers

Jason Oliver Fiering

Kirsty A McFarland

Jayson Linn Bowers

Robert Patrick Shivers

Nicole Billings

Gregory McKenzie

Peter Cavanagh

Brett Chevalier

Nicole E Raustad

Timothy Kuan Ta Lu

Helen Bartlett

Nicolas Mesyngier

Sarah Gruska

Lucas Tilley

Ken Kotz

Michael Sandor Koeris

Gregory John McKenzie

Timothy Kuan Lu

Brett Smith Chevalier

Gregory John Mckenzie

The present disclosure provides methods of generating recombinant bacteriophage genomes via semi-synthesis. Specifically, the present technology provides methods of integrating a heterologous nucleic acid sequence into a bacteriophage genome, and isolating recombinant bacteriophages that express the heterologous nucleic acid sequence.

Bacteriophage engineering via semi-synthesis

The present disclosure provides methods of generating recombinant bacteriophage genomes. Specifically, the present technology provides methods of integrating a heterologous nucleic acid sequence into a linear bacteriophage DNA genome, and isolating recombinant bacteriophages that express the heterologous nucleic acid sequence.

Phage engineering: protection by circularized intermediate

Systems and methods of testing a fluid sample are provided. A method can include controlling an orientation of a loader including a sample holder configured to hold a sample vessel to correspond to a first predetermined tilt angle. The method can include controlling an articulator to transfer the fluid sample from the sample vessel to at least one well of a plurality of wells of a multiwell plate positioned on a plate deck within the enclosure. The method can include controlling the articulator to move the multiwell plate to a hotel incubator within the enclosure. The method can include applying a bacteriophage to the fluid sample. The method can include controlling the articulator to move the multiwell plate from the hotel incubator to a testing system within the enclosure after a predetermined reaction time period. The method can include receiving output data from the testing system.

Automated bacteria identification and antibiotic susceptibility profiling device

A method for preparing and processing a sample is provided. The method includes obtaining a sample including biofluid. The method further includes purifying at least part of the sample via an acoustic separator to separate target cells from the sample. The sample may accordingly be at least partially purified. The method further includes causing a portion of an output collected from the acoustic separator to flow through a filter. At least one reagent, such as a lysis reagent or assay reagent, is caused to flow over the cells.

System and method of bacterial cell purification

The present disclosure provides compositions including recombinant K1E bacteriophages, methods for making the same, and uses thereof. The recombinant K1E bacteriophages disclosed herein are useful for the identification and/or antibiotic susceptibility profiling of specific bacterial strains/species present in a sample.

Composition of matter: engineering ofphage K1E

The system described herein for bacterial identification can be used as a point-of-care or lab-based diagnostic system. In some implementations, the system can be used to detect other foreign agents within blood or other samples. The system can include disposable microfluidic cartridges that are removable from the system. The microfluidic cartridges can receive a sample, such as a blood sample, that is suspected of containing bacterial cells and separate the bacterial cells from the blood sample. Once the bacterial cells are separated from the blood, the system can introduce the recombinant detector bacteriophages into the system that can infect the bacterial cells. The system can then detect the expression of reporter genes from the recombinant detector bacteriophages.

Bacteria identification and antibiotic susceptibility profiling device

The present disclosure provides compositions including recombinant B11 bacteriophages, methods for making the same, and uses thereof. The recombinant B11 bacteriophages disclosed herein are useful for the identification and/or antibiotic susceptibility profiling of specific bacterial strains/species present in a sample.

Recombinant B11 bacteriophages and uses thereof

The present disclosure provides methods and kits for generating recombinant bacteriophage genomes.

Bacteriophage engineering methods

The present disclosure provides methods and kits for generating recombinant bacteriophage genomes. Specifically, the present technology provides methods of integrating a heterologous nucleic acid sequence into a bacteriophage DNA genome, and isolating recombinant bacteriophages that express the heterologous nucleic acid sequence.

Bacteriophage recombination followed by blockage of non-recombinant bacteriophage replication

Methods of detecting target bacteria are provided. In some embodiments, the methods comprise exposing a sample to a phage capable of infecting target bacteria, said phage comprising a heterologous nucleic acid sequence encoding a marker. In some embodiments, the target bacteria comprise. In some embodiments, the target bacteria are all. Recombinantphage comprising a heterologous nucleic acid sequence encoding a marker are also provided as are useful combinations of such phage and articles of manufacture comprising such phage, among other things.

Recombinant phage for listeria detection

The present disclosure provides compositions including recombinant K or 812 bacteriophages, methods for making the same, and uses thereof. The recombinant K or 812 bacteriophages disclosed herein are useful for the identification and/or antibiotic susceptibility profiling of specific bacterial strains/species present in a sample.

Recombinant K and 812 bacteriophages and uses thereof

The present disclosure provides compositions including recombinant K1-5 bacteriophages, methods for making the same, and uses thereof. The recombinant K1-5 bacteriophages disclosed herein are useful for the identification and/or antibiotic susceptibility profiling of specific bacterial strains/species present in a sample.

Recombinant K1-5 bacteriophages and uses thereof

The present disclosure provides compositions including recombinant K2 bacteriophages, methods for making the same, and uses thereof. The recombinant K2 bacteriophages disclosed herein are useful for the identification and/or antibiotic susceptibility profiling of specific bacterial strains/species present in a sample.

Recombinant K2 bacteriophages and uses thereof

The present disclosure provides compositions including recombinant PB1 bacteriophages, methods for making the same, and uses thereof. The recombinant PB1 bacteriophages disclosed herein are useful for the identification and/or antibiotic susceptibility profiling of specific bacterial strains/species present in a sample.

Growing community of inventors

Somerville, MA, United States of America

Jason Wyatt Holder