Oxford Nanopore Technologies Limited

Sharp Life Science (eu) Limited

Jason Robert Hyde

Andrew John Heron

Clive Gavin Brown

James Anthony Clarke

Paul Raymond Mackett

Pedro Miguel Ortiz Bahamon

Gaëlle Anne-Leonie Andreatta

James White

Benjamin James Hadwen

Sally Anderson

Gurdial Singh Sanghera

Matthew Holden

Justin Millis

Ken Healy

Mark David Jackson

Ping Xie

Jonathan Edward McKendry

Richard Kenneth John Wiltshire

Mark Hyland

Rhodri Rhys Davies

Robert Greasty

Ioana Pera

An array of membranes comprising amphipathic molecules is formed using an apparatus comprising a support defining an array of compartments. Volumes comprising polar medium are provided within respective compartments and a layer comprising apolar medium is provided extending across the openings with the volumes. Polar medium is flowed across the support to displace apolar medium and form a layer in contact with the volumes, forming membranes comprising amphipathic molecules at the interfaces. In one construction of the apparatus, the support that comprises partitions which comprise inner portions and outer portions. The inner portions define inner recesses without gaps therebetween that are capable of constraining the volumes comprising polar medium contained in neighbouring inner recesses from contacting each other. The outer portions extend outwardly from the inner portions and have gaps allowing the flow of an apolar medium across the substrate.

Formation of array of membranes and apparatus therefor

Droplet interfaces are formed between droplets in an electro-wetting device comprising an array of actuation electrodes. Actuation signals are applied to selected actuation electrodes to place the droplets into an energised state in which the shape of the droplets is modified compared to a shape of the droplets in a lower energy state and to bring the two droplets into proximity. The actuation signals are then changed to lower the energy of the droplets into the lower energy state so that the droplets relax into the gap and the two droplets contact each other thereby forming a droplet interface. The use of sensing electrodes in the device permit electrical current measurements across the droplet interface. The sensing electrodes can be used for either (i) applying a reference signal during droplet actuation or (ii) recording electrical current measurements.

Droplet interfaces in electro-wetting devices

An apparatus for supporting an array of layers of amphiphilic molecules, the apparatus comprising: a body, formed in a surface of the body, an array of sensor wells capable of supporting a layer of amphiphilic molecules across the sensor wells, the sensor wells each containing an electrode for connection to an electrical circuit, and formed in the surface of the body between the sensor wells, flow control wells capable of smoothing the flow of a fluid across the surface.

Apparatus for supporting an array of layers of amphiphilic molecules and method of forming an array of layers of amphiphilic molecules

A detachable electrical device can be formed from a kit comprising a pair of component parts adapted for connection to each other, wherein the connected components of the device may be subsequently disconnected, comprising: an array of electrical connectors, each electrical connector comprising an electrically conductive liquid; and an array of electrodes; wherein the arrays can be brought into contact with each other so as to provide a plurality of electrical connections between the electrically conductive liquid of the array of electrical connectors and the electrodes of the array of electrodes, and wherein the electrical connections may be subsequently broken by detaching the electrically conductive liquid from the electrodes of the array.

Electrical device with detachable components

An apparatus for supporting an array of layers of amphiphilic molecules, the apparatus comprising: a body (), formed in a surface of the body, an array of sensor wells () capable of supporting a layer of amphiphilic molecules () across the sensor wells, the sensor wells each containing an electrode () for connection to an electrical circuit, and formed in the surface of the body between the sensor wells, flow control wells capable of smoothing the flow of a fluid across the surface.

The invention provides a method of forming a membrane between a first volume of polar medium and a second volume of polar medium. In some embodiments, the method involves providing a first volume including polar medium and a second volume including polar medium which are separated from one another by an apolar medium, in which at least one of the first and second volumes has a layer including amphipathic molecules.

Droplet interfaces

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Oxford, United Kingdom

Jason Robert Hyde