Becton Dickinson and Company Limited

Jason P Stevens

Danielle Koffenberger

Courtney E Maus

The present invention relates to nucleic acid amplification assays for the detection of nucleic acid sequences of. The present invention provides oligonucleotides that are complementary or that anneal to nucleic acid sequences of the vly gene of GV. The present invention also provides internal amplification controls (IACs) that can be used in nucleic acid amplification reactions.

Gardnerella vaginalis assay

A region of thepmpA gene has been identified which is useful for performing amplification assays to determine specifically whetheris present in the sample being tested. Oligonucleotides useful for detecting this gene by performing the polymerase chain reaction (PCR) are disclosed. The disclosed oligonucleotides can be used in an assay which is specific for multiple strains or serovars of, including the variant E serovar, and which does not show cross reactivity with the genomes of other microorganisms or with human DNA. In addition, the disclosed oligonucleotides can be used to in a multiplex system. This invention also contemplates a kit including oligonucleotides, and optionally other reagents, for the detection ofusing PCR.

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Assay for Chlamydia trachomatis by amplification and detection of  Chlamydia trachomatis  pmpA gene

The present invention relates to nucleic acid amplification assays for the detection of nucleic acid sequences of Gardnerella vaginalis. The present invention provides oligonucleotides that are complementary or that anneal to nucleic acid sequences of the vly gene of GV. The present invention also provides internal amplification controls (IACs) that can be used in nucleic acid amplification reactions.

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Owings Mills, MD, United States of America

Jason P Stevens