Micromass UK Limited

Leco Corporation

Micromss UK Limited

Miromass UK Limited

Jason Lee Wildgoose

Martin Raymond Green

Kevin Giles

Steven Derek Pringle

David John Langridge

Keith George Richardson

Daniel James Kenny

Robert Harold Bateman

John Brian Hoyes

Jeffery Mark Brown

Anthony James Gilbert

Farnoush Salarzaei

Richard Denny

Richard Barrington Moulds

Steve Pringle

Peter Carney

Kevin R Howes

Soji Chummar

Michael Raymond Morris

Anatoly Verenchikov

James Ian Langridge

Mikhail Yavor

Jeffrey Mark Brown

Paul McIver

Garry Michael Scott

David Wallis

Dipesh Mistry

George Andreas Antoniades

Cedric Marsh

Nicholas Tomczyk

Marc Victor Gorenstein

David Gordon

Paul Robert Murray

Gareth Rhys Jones

John Skilling

Kate Whyatt

Peter Gerard Spreadbury

Ruth Wamsley

Oliver John Malpas

David Darrell Williams

Jakub Ujma

Peter Nixon

Eleanor Riches

Kevil Giles

Martin Palmer

Darren Hewitt

Matthew Collier

Keith Richardson

A method of selecting ions comprises selecting ions corresponding to a target ion of interest by separating analyte ions according to their ion mobility, isolating first ions of the analyte ions, separating the first ions according to their ion mobility, and isolating second ions of the first ions. Preferably, the separation is accomplished by using a cyclic or closed-loop separator.

Method of selecting ions by ion mobility separation and isolation of ions

An ion guide assembly () is disclosed comprising: two planar mounting components (); and first and second ion guides () mounted on the two planar mounting components such that the ion guides are spaced apart from each other, wherein at least one of the planar mounting components has an aperture () therethrough that is located between the positions on said at least one mounting component at which the first and second ion guides are mounted; and an ion optical device sized and configured to be inserted through the aperture in the planar mounting component and into the space between the first and second ion guides.

Ion guide assembly having multiple ion guides

An analytical device for analysing ions is provided comprising a separatorfor separating ions according to a physico-chemical property and an interfacecomprising one or more ion guides. A quadrupole rod set mass filteris arranged downstream of the interface. A control system is arranged and adapted: (i) to transmit a first group of ions which emerges from the separatorthrough the interfacewith a first transit time t1 and

Optimized ion mobility separation timescales for targeted ions

A method of mass spectrometry is disclosed comprising: a step () of analysing a reference compound in a first mass spectrometer and outputting mass spectral data in response thereto; a step () of analysing the reference compound in a second, different mass spectrometer and outputting mass spectral data in response thereto; and a step () of automatically adjusting an operational parameter, duty cycle (e.g. duty cycle of data acquisition), or acquired spectral data of at least one mass spectrometer such that, for the same (given) consumption of reference compound by the spectrometer, the statistical precision of quantification (the number of detected ions) and/or of mass measurement (the mass resolution) by the mass spectrometer is substantially the same as that of the other mass spectrometer. A similar method of ion mobility spectrometry is disclosed.

Automatically standardising spectrometers

A method of analysing ions is disclosed comprising: (i) subjecting ions of an analyte molecule to different activation levels at different times so as to cause the ions to have different mobilities at said different times, wherein the activation level is varied in a plurality of cycles, and wherein the activation level is varied between said different levels during each of the cycles. The method uses an ion mobility separator or scanned ion mobility filter to determine the mobilities of the ions for said different activation levels; and correlates the determined mobilities with their respective activation levels so as to thereby obtain a fingerprint for the analyte molecule.

Ion identification using ion mobility spectrometry

A method of time of flight (ToF) mass spectrometry comprising: pushing ions into a ToF mass analyser in a plurality of pushes, wherein the time spacing between adjacent pushes is shorter than either the longest flight time, or the range of flight times, of the ions; detecting the ions so as to obtain spectral data; decoding the spectral data to determine first mass spectral data relating to ions pushed into the ToF mass analyser by a first plurality of the pushes (P-P), and allocating this first mass spectral data to a first time stamp (t); and decoding the spectral data to determine second mass spectral data relating to ions pushed into the ToF mass analyser by a second plurality of the pushes (P-P), and allocating this second mass spectral data to a second time-stamp (t); wherein the first and second time-stamps have a time difference therebetween that is shorter than said longest flight time, or said range of flight times (), in the ToF mass analyser.

Multiplexed time of flight mass spectrometer

A method of mass spectrometry is disclosed comprising performing a plurality of experimental runs, wherein each experimental run comprises: periodically mass analysing fragment or product ions at a plurality of time intervals, wherein a delay time is provided between the start of the experimental run and the first time interval at which the fragment or product ions are mass analysed. Different delay times are provided in different ones of the experimental runs and fragment or product ions that have been analysed in the same time interval in at least one of said experimental runs and that have been analysed in different time intervals in at least one other of said experimental runs are identified as fragment or product ions of interest. These fragment or product ions are thus determined to relate to different precursor ions and are used to identify their respective precursor ions.

Two dimensional MS/MS acquisition modes

A mass spectrometer comprising: a vacuum housing comprising a first vacuum chamber having a first gas exhaust port; a gas pump () having a first gas inlet port connected to the first gas exhaust port (H) by a first gas conduit for evacuating the first vacuum chamber, and a first apertured cover () arranged over the first gas exhaust port (H) or first gas inlet port, or in the first gas conduit therebetween.

Bench-top time of flight mass spectrometer

A method is disclosed comprising: trapping ions in an ion trap (); applying a first force on the ions within the ion trap in a first direction, said force having a magnitude that is dependent upon the value of a physicochemical property of the ions; applying a second force on these ions in the opposite direction so that the ions separate according to the physicochemical property value as a result of the first and second forces; and then pulsing or driving ions out of one or more regions of the ion trap.

Separating ions in an ion trap

A method of mass spectrometry is disclosed comprising: performing a plurality of cycles of operation during a single experimental run, wherein each cycle comprises: mass selectively transmitting precursor ions of a single mass, or range of masses, through or out of a mass separator or mass filter at any given time, wherein the mass separator or mass filter is operated such that the single mass or range of masses transmitted therefrom is varied with time; and mass analysing ions.

Two dimensional MSMS

A method of mass spectrometry is disclosed comprising: a) providing temporally separated precursor ions; b) mass analyzing separated precursor ions, and/or product ions derived therefrom, during a plurality of sequential acquisition periods, wherein the value of an operational parameter of the spectrometer is varied during the different acquisition periods; c) storing the spectral data obtained in each acquisition period along with its respective value of the operational parameter; d) interrogating the stored spectral data and determining which of the spectral data for a precursor ion or product ions meets a predetermined criterion, and determining the value of the operational parameter that provides this mass spectral data as a target operational parameter value; and e) mass analyzing again the precursor or product ions whilst the operational parameter is set to the target operational parameter value.

Optimised targeted analysis

A method of introducing and ejecting ions from an ion entry/exit device () is disclosed. The ion entry/exit device () has at least two arrays of electrodes (). The device is operated in a first mode wherein DC potentials are successively applied to successive electrodes of at least one of the electrode arrays (() in a first direction such that a potential barrier moves along the at least one array in the first direction and drives ions into and/or out of the device in the first direction. The device is also operated in a second mode, wherein DC potentials are successively applied to successive electrodes of at least one of the electrode arrays () in a second, different direction such that a potential barrier moves along the array in the second direction and drives ions into and/or out of the device in the second direction. The device provides a single, relatively simple device for manipulating ions in multiple directions. For example, the device may be used to load ions into or eject ions from an ion mobility separator in a first direction, and may then be used to cause ions to move through the ion mobility separator in the second direction so as to cause the ions to separate.

Ion entry/exit device

A Time of Flight analyser comprising a flight tube () and a reflectron (), wherein the reflectron comprises a stack of electrodes () that are compressed against the flight tube such that they remain parallel to each other under compression.

Bench-top Time of Flight mass spectrometer

A mass spectrometer comprising: a vacuum housing comprising a first vacuum chamber having a first gas exhaust port; a gas pump () having a first gas inlet port connected to the first gas exhaust port (H) by a first gas conduit for evacuating the first vacuum chamber; and a first apertured cover () arranged over the first gas exhaust port (H) or first gas inlet port, or in the first gas conduit therebetween.

A method of encoding a parent or precursor ion beam to aid product ion assignment is disclosed. According to an embodiment the energy of parent ions entering a collision cellis progressively increased. Different species of parent ions fragment at different collision energies. Fragment ion intensity profiles are matched with parent ion intensity profiles to correlate fragment ions with corresponding parent ions.

Encoding of precursor ion beam to aid product ion assignment

Growing community of inventors

Stockport, United Kingdom

Jason Lee Wildgoose