Iti Scotland Limited

Jason Kelly

Reshma P Shetty

Austin Che

Tom Knight

Barry Canton

Maiwenn Kersaudy Kerhoas

Farid Amalou

Will Shu

Kersaudy Kerhoas Maiwenn,

Provided herein are methods for the assembly of a polynucleic acid sequence that is at least partially carried out on a microfluidic device; methods for the preparation of a library of polynucleic acid sequences; microfluidic devices; methods for designing nucleic acid sequences; methods for planning the assembly of a polynucleic acid sequence from a plurality of nucleic acid sequences; systems comprising components for carrying out these methods; computer programs which, when run on a computer, implements these methods; and computer readable medium or carrier signals encoding such a computer program.

Method for the assembly of a polynucleic acid sequence

The present invention provides a method for the assembly of a polynucleic acid sequence from a plurality of nucleic acid sequences in which the polynucleic acid sequence is of a formula N, in which N represents a nucleic acid sequence and where n is 1 or greater than 1 and each N may be the same or a different nucleic acid sequence, in which the method comprises: (i) providing a first nucleic acid sequence N1 which has an oligonucleotide linker sequence L1at the 3'-end of the nucleic acid sequence; (ii) providing a second nucleic acid sequence N2 which optionally has an oligonucleotide linker sequence L2at the 3&#x2032;-end of the nucleic acid sequence and which has an oligonucleotide linker sequence L2at the 5&#x2032;-end of the nucleic acid sequence, wherein the 5&#x2032;-end linker sequence L2of nucleic acid sequence N2 is complementary to the 3&#x2032;-end linker sequence L1of nucleic acid sequence N1; (iii) optionally providing one or more additional nucleic acid sequences N, wherein nucleic acid sequence N2 has an oligonucleotide linker sequence L2at the 3&#x2032;-end of the nucleic acid sequence, and wherein said one or more additional nucleic acid sequences N comprises a terminal additional nucleic acid sequence NZ, and wherein each additional nucleic acid sequence N has an oligonucleotide linker sequence at its 3&#x2032;-end, wherein said terminal additional nucleic acid sequence NZ optionally lacks an oligonucleotide linker sequence at its 3&#x2032;-end and wherein each additional nucleic acid sequence N has an oligonucleotide linker sequence at its 5&#x2032;-end, wherein for the first additional nucleic acid sequence N3 the 5&#x2032;-end linker sequence L3is complementary to the 3&#x2032;-end linker sequence L2of nucleic acid sequence N2 and for each second and subsequent additional nucleic acid sequence N the 5&#x2032;-end linker sequence is complementary to the 3&#x2032;-end linker sequence of the respective preceding additional nucleic acid sequence; and (iv) ligating said nucleic acid sequences to form said polynucleic acid sequence.

Method for assembly of polynucleic acid sequences

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Boston, MA, United States of America

Jason Kelly