University of Chicago

University of Washington

California Institute of Technology

Jason E Kreutz

Rustem F Ismagilov

Daniel T Chiu

Feng Shen

Wenbin Du

Bryant S Fujimoto

Bing Sun

Gloria S Yen

Delai Chen

Liang Li

Kevin Paul Flood Nichols

Jiangbo Yu

Travis S Schlappi

Stephanie McCalla

Weishan Liu

Jiasi Wang

Toan Huynh

Justin Rolando

Thomas Schneider

Shencheng Ge

Methods, devices, and systems for performing digital assays are provided. In certain aspects, the methods, devices, and systems can be used for the amplification and detection of nucleic acids. In certain aspects, the methods, devices, and systems can be used for the recognition, detection, and sizing of droplets in a volume. Also provided are compositions and kits suitable for use with the methods and devices of the present disclosure.

Methods and systems for performing digital assays using polydisperse droplets

Methods, devices, and systems for performing digital assays are provided. In certain aspects, the methods, devices, and systems can be used for the detection of nucleic acids and proteins. Also provided are methods, devices, systems, and compositions for improved detection and quantification of target molecules using encoded probes. In certain aspects, the methods, devices, and systems provided herein are useful in multiplexed digital assays. In certain aspects, the methods, devices, and systems can be used for the recognition, detection, and sizing of compartmentalized volumes in a volume. Also provided are compositions and kits suitable for use with the methods and devices of the present disclosure.

Digital nucleic acid amplification using encoded particles

Systems, methods, and devices for discretizing and analyzing fluidic samples are provided. In one aspect, a microfluidic array for discretizing a fluidic sample comprises one or more flow channels and a plurality of fluidic compartments in fluidic communication with the one or more flow channels. In another aspect, a system for discretizing and analyzing fluidic samples comprises a rotor assembly shaped to receive a microfluidic device.

Systems, methods, and devices for self-digitization of samples

Devices, systems and apparatuses for the discretization and manipulation of sample volumes are provided. Related methods are also provided.

Self-digitization of sample volumes

Methods and apparatus for performing digital assays using polydisperse droplets

Provided herein are methods, compositions, and devices for the identification and quantification of analytes, such as nucleic acids, proteins, cells or other biological samples. The methods, compositions, and devices are suited for accurate, portable analysis of small amounts of analyte.

Digital assay for quantifying and concentrating analytes

A device is described having a first surface having a plurality of first areas and a second surface having a plurality of second areas. The first surface and the second surface are opposed to one another and can move relative to each other from at least a first position where none of the plurality of first areas, having a first substance, are exposed to plurality of second areas, having a second substance, to a second position. When in the second position, the plurality of first and second areas, and therefore the first and second substances, are exposed to one another. The device may further include a series of ducts in communication with a plurality of first second areas to allow for a substance to be disposed in, or upon, the plurality of second areas when in the first position.

Slip chip device and methods

Provided are devices and methods for effecting processing of samples, including essentially isothermal amplification of nucleic acids, at multiple reaction locations in a single device. In some embodiments, the disclosed devices and methods provide for effecting parallel sample processing in several hundred locations on a single device.

Analysis devices, kits, and related methods for digital quantification of nucleic acids and other analytes

Provided are devices comprising multivolume analysis regions, the devices being capable of supporting amplification, detection, and other processes. Also provided are related methods of detecting or estimating the presence nucleic acids, viral levels, and other biological markers of interest.

Multivolume devices, kits and related methods for quantification and detection of nucleic acids and other analytes

Multivolume devices, kits and related methods for quantification of nucleic acids and other analytes

Growing community of inventors

Seattle, WA, United States of America

Jason E Kreutz