Genzyme Corporation

Jason C Goodrick

Nick C Wan

This invention relates to a serum-free eukaryotic cell culture medium supplement. The supplement comprises carbon sources, vitamins, inorganic salts, amino acids and a protein digest. The medium supplement of the present invention enables the maintenance of mammalian cell cultures at cell densities equal to or greater than that obtained with batch culture methods while increasing longevity and productivity.

Serum-free medium supplement

This invention relates to an aqueous medium formulation which is capable of allowing the growth of a plasmid-producing host cell to a density greater than 30 at a reading of 600 nm and, in addition, enhancing plasmid production beyond that which would be expected at the cell densities achieved. The medium of the present invention comprises a carbon source in the concentration range of about 20 to about 40 grams per liter of the water component of the medium.

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Cambridge, MA, United States of America

Jason C Goodrick