Illumina Corporation

Illumina Cambridge Limited

Solexa, Inc.

Jason Bryant

Dale Buermann

Wenyi Feng

Mark R Pratt

Harold Philip Swerdlow

Bojan Obradovic

Mark T Reed

Steven Mark Barnard

Maria Candelaria Rogert Bacigalupo

Mark Allen Osborne

Stanley S Hong

Colin Lloyd Barnes

Mark Wang

Erik Lewis Williamson

Drew R Verkade

Eric Hans Vermaas

Bryan Crane

Jason Harris

Patrick Leung

Klaus Maisinger

Frederick G Erie

Alexander Kindwall

Andrew James Carson

Isabelle Rasolonjatovo

Alexander P Kindwall

Saibal Banerjee

Subra Sankar

Chiara Rodighiero

Peter Lundberg

Xavier Lee

Johnny Ho

Sergey Etchin

Kevin Benson

Eric Williamson

Frederick Erie

Jonny Ho

Ning Sizto

A system and method for imaging biological samples on multiple surfaces of a support structure are disclosed. The support structure may be a flow cell through which a reagent fluid is allowed to flow and interact with the biological samples. Excitation radiation from at least one radiation source may be used to excite the biological samples on multiple surfaces. In this manner, fluorescent emission radiation may be generated from the biological samples and subsequently captured and detected by detection optics and at least one detector. The detected fluorescent emission radiation may then be used to generate image data. This imaging of multiple surfaces may be accomplished either sequentially or simultaneously. In addition, the techniques of the present invention may be used with any type of imaging system. For instance, both epifluorescent and total internal reflection methods may benefit from the techniques of the present invention.

Compensator for multiple surface imaging

A fluidic device holder configured to orient a fluidic device. The device holder includes a support structure configured to receive a fluidic device. The support structure includes a base surface that faces in a direction along the Z-axis and is configured to have the fluidic device positioned thereon. The device holder also includes a plurality of reference surfaces facing in respective directions along an XY-plane. The device holder also includes an alignment assembly having an actuator and a movable locator arm that is operatively coupled to the actuator. The locator arm has an engagement end. The actuator moves the locator arm between retracted and biased positions to move the engagement end away from and toward the reference surfaces. The locator arm is configured to hold the fluidic device against the reference surfaces when the locator arm is in the biased position.

Systems, methods, and apparatuses to image a sample for biological or chemical analysis

A detection system including a detection assembly to receive emission light emitted from a sample. The detection assembly has a multi-band dichroic member and at least first and second detection devices. The multi-band dichroic member has a transmission/reflection characteristic with first and second transmissive regions and a reflective region along a wavelength spectrum. The dichroic member transmits portions of the emission light that align with the first and second transmissive regions and reflects a portion of the emission light that aligns with the reflective region. The dichroic member includes a single mirror with an incident surface. The incident surface is configured to transmit the portions of the emission light that align with the first and second transmissive regions and configured to reflect the portion of the emission light that aligns with the reflective region.

Fluorescence excitation and detection system and method

A detection system for separately detecting different wavelengths of emission light emitted from a sample. The system includes a detection assembly to receive the emission light emitted from the sample. The detection assembly includes a multi-band dichroic member and at least first and second detection cameras. The multi-band dichroic member has a transmission/reflection characteristic with at least two transmissive regions separated from each other along a wavelength spectrum and with at least one reflective region separated from the transmissive regions along the wavelength spectrum. The dichroic member transmits portions of the emission light that align with the at least two transmissive regions to the first detection camera. The dichroic member reflects a portion of the emission light that aligns with the reflective region to the second detection camera.

The present invention comprises systems and devices for sequencing of nucleic acid, such as short DNA sequences from clonally amplified single-molecule arrays.

Systems and devices for sequence by synthesis analysis

Growing community of inventors

Essex, United Kingdom

Jason Bryant