10x Genomics, Inc.

10x Gemomics, Inc.

Jason Bell

Francesca Meschi

Michael Schnall-Levin

Xinying Zheng

Geoffrey McDermott

Zahra Kamila Belhocine

Katherine Pfeiffer

Jerald Sapida

Katrina Sullivan-Bibee

Corey Nemec

Provided herein are methods, compositions, and systems for transposon loading. Transposons are loaded with nucleic acid molecules, allowing for transposition reactions of cellular nucleic acids. The present invention may improve transposon loading, yield of productive fragments, while minimizing potential nucleic acid fragment loss or cross-contamination.

Systems and methods for transposon loading

Methods and compositions to minimize barcode exchange during the preparation of barcoded next-generation sequencing libraries prepared from a single cell. The methods utilize oligonucleotides containing a 3'-terminated blocking group or sequences that prevent amplification or extension.

Methods and systems to minimize barcode exchange

Provided herein are compositions and methods for cellular analysis. Nucleic acid from single cells may be processed in one or more partitions. A partition may comprise a cell bead and one or more enzymes for nucleic acid processing. A partition may comprise a functionalized polymer. In some cases, single cells may be subjected to epigenetic analysis, thereby generating an epigenetic profile for each cell from a plurality of cells.

Compositions and methods for cellular processing

The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization from a single cell. Such polynucleotide processing may be useful for a variety of applications. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins) and chromatin (e.g., accessible chromatin).

Methods and systems for analysis of chromatin

The present disclosure provides methods, systems, and kits for processing nucleic acid molecules. A method may comprise providing a template nucleic acid fragment (e.g., within a cell, cell bead, or cell nucleus) within a partition (e.g., a droplet or well) and subjecting the template nucleic acid fragment to one or more processes including a barcoding process and a single primer extension or amplification process. The processed template nucleic acid fragment may then be recovered from the partition and subjected to further amplification to provide material for subsequent sequencing analysis. The methods provided herein may permit simultaneous processing and analysis of both DNA and RNA molecules originating from the same cell, cell bead, or cell nucleus.

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Palo Alto, CA, United States of America

Jason Bell