Anadys Pharmaceuticals, Inc.

Jason A Thresher

C Richard Wobbe

Judith M Healy

Yueh-Tyng Chien

Patricia Carroll

The present invention provides an isolated Staphylococcal GTPase obg gene and a Staphylococcal GTP-binding protein encoded thereby. A recombinant expression vector and an engineered host cell are also provided that include a polynucleotide encoding a Staphylococcal GTP-binding protein. The present invention further relates to a method of producing Staphylococcal GTP-binding protein and a method for high throughput screening to identify potential antimicrobial compounds useful against Staphylococcal bacterial strains.

Staphylococcal GTPase obg nucleotide sequence encoding Staphylococcal GTP-binding protein

The present invention relates to a recombinant organism, and a bacterial strain such as Staphylococcus, and , in particular. The organism has a regulatable gene for encoding an RNA polymerase specificity factor required for expression of at least one gene essential for growth of the organism. The regulatable gene is one which is responsive to an exogenous effector molecule, and may in particular be an inducer-responsive gene including an operator site, such as a lac operator, to which a repressor, such as a lacl-encoded repressor, is capable of binding. The regulatable gene may in particular be an IPTG responsive plaC allele.

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Somerville, MA, United States of America

Jason A Thresher