Pioneer Hi-bred International, Inc.

E.i. Dupont De Nemours and Company

Corteva Agriscience LLC

Jasdeep Mutti

Andrew Mark Cigan

Christopher Jay Scelonge

Huirong Gao

Dean Podlich

Zhan-Bin Liu

Zhan-bin Liu

Compositions and methods are provided for introducing transgenic target sites for Site Specific Integration (SSI) and/or polynucleotides of interest into at least one double-strand break target site of a double-strand-break inducing agent in a genomic window of a plant genome. Also provided are methods and compositions for producing a complex trait locus in a genomic window of a plant comprising at least one transgenic target site for site specific integration integrated in at least double-strand-break target site. The double-strand-break target site can be, but is not limited to, a target site for a zinc finger endonuclease, an engineered endonuclease, a meganuclease, a TALENs and/or a Cas endonuclease. The genomic window of said plant can comprise at least one genomic locus of interest such as a trait cassette, a transgene, a mutated gene, a native gene, an edited gene or a site-specific integration (SSI) target site.

Generation of site specific integration sites for complex trait loci in corn and soybean, and methods of use

Generation of site-specific-integration sites for complex trait loci in corn and soybean, and methods of use

Growing community of inventors

Johnston, IA, United States of America

Jasdeep Mutti