Ceskoslovenska Akademie Ved

Jaroslav Rosol

Bedrich Porsch

Jaroslav Voslar

Vladimir Kubanek

A column for preparative liquid chomatography is provided with an inlet tube (5) for injected sample, which is closed on the end with a gauze or fritted plate (6), passes through the center of a lower terminal (3), and ends in the distance equal to 70 to 90% of the total length of the column from the lower end of this tube, whereas the lower terminal (3) has at least four openings (7) or a circular slot or two half-slots which are placed outside the center of terminal on a concentric circle with diameter ranging between 40 to 70% of the inner diameter of column tube (1). The column operates in such a way, that mobile phase is introduced into the column through the center of the upper terminal (2) and a sample is injected into a sorbent through the inlet tube (5) for sample after stopping the flow of mobile phase and then the flow of mobile phase is restored.

Column for preparative liquid chromatography

The invented column for liquid chromatography consists of a glass tube, which is inserted into a metallic tube. The metallic tube is provided with recesses in its inner wall at both ends, which accommodate conic tightening rings from metal together with conic circumferential packings from plastics, advantageously from poly(tetrafluoroethylene). The column further consists of front packings from plastics, advantageously from poly(tetrafluoroethylene, which have porous disks or filter gauzes pressed into their recesses. Both types of packing are tightened with metallic terminal elements screwed on the metallic tube, which are provided with expansion slots at sides adjacent to the front packings. The metallic tube may have openings for a visual inspection of column packing.

Growing community of inventors

Prague, Czechia

Jaroslav Rosol