Glykos Finland Oy

Nutrileads B.v.

Suomen Punainen Risti Veripalvelu

Other

Novartis International Pharmaceutical, Ltd.

Vtt Technical Research Centre of Finland Ltd

Glykos Finland

Nestec S.a.

Jari Natunen

Juhani Saarinen

Tero Satomaa

Markku Saloheimo

Anne Huuskonen

Anne Kanerva

Annamari Heiskanen

Heidi Anderson

Ann Westerholm-Parvinen

Christopher Landowski

Maria Blomqvist

Jarmo Laine

Ulla Impola

Anna-Liisa Hänninen

Anne Olonen

Noora Salovuori

Taina Jaatinen

Jari Helin

Jukka Hiltunen

Johanna Nystedt

Merja Elisa Penttila

Jean Hypolites Koek

Merja Penttilä

Ruud Albers

Erwin Werner Tareilus

Anita Laitinen

Milla Mikkola

Werner Klaffke

Petronella Anna Kreijveld

Richardus Paulus Anton Oranje

Jukka Partanen

Christian Ostermeier

Benjamin Patrick Sommer

Sari Tiitinen

Ramon Wahl

Heli Viskari

Bernhard Helk

Leena Valmu

Hanna Salo

Karl-Anders Karlsson

Anne Leppänen

Jussi Joensuu

Heidi Salminen

Anja Vilkman

Heidi Virtanen

Georg Schmidt

Ritva Niemela

Eero Mustalahti

Minna Tiittanen

Susann Teneberg

Sarl Tiitinen

Juha Natunen

Halina Miller-Podraza

Merja Penttila

Anna-Liisa Hanninen

Ritva NiemelÄ

Jonas Angström

Maan Abul-Milh

Niamh Roche

The present disclosure relates to compositions and methods useful for the production of heterologous proteins in filamentous fungal cells.

Protease deficient filamentous fungal cells and methods of use thereof

The present disclosure relates to compositions and methods useful for the production of heterologous proteins with reduced O-mannosylation in filamentous fungal cells, such ascells. More specifically, the invention provides a PMT-deficient filamentous fungal cell comprising a) at least a first mutation that reduces an endogenous protease activity compared to a parental filamentous fungal cell which does not have said first mutation, and, b) at least a second mutation in a PMT gene that reduces endogenous O-mannosyltransferase activity compared to a parental filamentous fungal cell which does not have said second mutation, wherein said filamentous fungal cell is selected from the group consisting oforcell.

O-mannosyltransferase deficient filamentous fungal cells and methods of use thereof

The present disclosure relates to compositions and methods useful for the production of recombinant glycoproteins in filamentous fungal cells, such ascells, wherein at least 90% (mol %), preferably at least 95% of the total neutral N-glycans of said produced recombinant glycoprotein are mammalian-like N-glycans. More specifically, the invention provides a filamentous fungal cell comprising i. one or more mutations that reduces or eliminates one or more endogenous protease activity compared to a parental filamentous fungal cell which does not have said mutation(s); ii. a polynucleotide encoding a heterologous catalytic subunit of oligosaccharyl transferase; iii. a recombinant polynucleotide for increasing &#x3b1;1, 2 mannosidase activity;and, iv. a recombinant polynucleotide encoding said heterologous glycoprotein.

Production of glycoproteins with mammalian-like N-glycans in filamentous fungi

Objective of the present invention is to provide polysaccharides which modulate immune response, and which can be used as ingredients in edible products or pharmaceutical compositions. The present invention provides such polysaccharides obtained from the species, which comprise a rhamnogalacturonan-I core, and wherein the molar ratio of galacturonyl acid residues to rhamnosyl residues in the backbone of the polysaccharide is close to 1:1. The present invention also provides edible products or pharmaceutical compositions containing such polysaccharides, in order to modulate immune response.

Polysaccharide suitable to modulate immune response

The invention describes specific sialylated structures present on human stem cells and cell populations derived thereof. The invention is especially directed to methods to control the status of stem cells by observing changes in sialylation of the cells; and control of potential contaminations of biological materials; and reagents and methods used in connection with the cells in order to avoid alterations of the cell glycosylation by contaminating materials. The invention is further directed to novel stem cells, the glycosylation of which has been specifically altered.

Method for evaluating cell populations

Described herein are compositions including filamentous fungal cells, such asfungal cells, having reduced protease activity and expressing fucosylation pathway. Further described herein are methods for producing a glycoprotein having fucosylated N-glycan, using genetically modified filamentous fungal cells, for example,fungal cells, as the expression system.

Production of fucosylated glycoproteins

The invention relates to a composition comprising a glycoprotein comprising the Fc domain of an antibody, or a fragment thereof, comprising an Asn (asparagine) residue and an oligosaccharide structure attached thereto, wherein said oligosaccharide structure has a structure according to formula I; and wherein at least 20% of the oligosaccharide structures attached to glycoprotein in the composition consist of oligosaccharide structures according to formula (I).

Glycoprotein

The invention describes methods for production of novel composition of glycans, glycomes, from human multipotent stem cells. The invention is further directed to methods for modifying the glycomes and analysis of the glycomes and the modified glycomes. Furthermore the invention is directed to stem cells carrying the modified glycomes on their surfaces.

Carbohydrate profile compositions from human cells and methods for analysis and modification thereof

The present disclosure relates to recombinant proteins having N-acetylglucosaminyltransferase activity. The present disclosure further relates to methods for producing complex N-glycans including the steps of providing host cells containing such recombinant proteins and culturing the host cells such that the recombinant proteins are expressed.

Fusion enzymes

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Vantaa, Finland

Jari Natunen