Wallac Oy

Walloc Oy

Jari Hovinen

Veli-Matti Mukkala

Jari Peuralahti

Harri Hakala

Harri Takalo

Lassi Jaakkola

Päivi Liitti

Heikki Mikola

Janne Ketola

This invention relates to a group of novel chelating agents, novel chelates, biomolecules labeled with said chelates or chelating agents as well as solid supports conjugated with said chelates, chelating agents or labeled biomolecules. Especially the invention relates to novel chelating agents useful in solid phase synthesis of oligonucleotides or oligopeptides and the oligonucleotides and oligopeptides so obtained.

Luminescent lanthanide (III) chelates, chelating agents and conjugates derived thereof

This invention concerns novel labeling reactants based on azacycloalkanes, wherein a suitable group is linked to the molecule allowing introduction of the said molecules to bioactive molecules in solution or on solid phase.

Biomolecule labeling reactants based on azacycloalkanes and conjugates derived thereof

Chelating agents and highly luminescent and stable chelates and their use

This invention concerns novel terminating substrates for DNA polymerases. The substrates are nucleoside triphosphates labeled with lanthanide chelates.

Terminating substrates for DNA polymerases

This invention concerns novel labeling reactants suitable for labeling of a biospecific binding reactant using solid-phase synthesis. The novel reactants are derivatives of diethylenetriaminepentaacetic acid (DTPA), wherein a suitable group is linked to the DTPA molecule, thus allowing site specific introduction of the ligand of the derivatives to bioactive molecules on solid phase in an oligopeptide synthesizer.

Labeling reactant

The invention relates to a labeling reactant of formula (I) useful for labeling an oligonucleotide wherein: R is a temporary protecting group. A is either a phosphorylating moiety or a solid support tethered to Z via a linker arm E&#x2033;. Z is a bridge point and is formed from E is a linker arm between R and Z. E' is a linker arm between Z and Z&#x2032;. E&#x2033; is a linker arm between Z and A. E&#x2032;&#x2033; is a linker arm between Z&#x2032; and G. Z&#x2032; is a purine or pyrimidine base. G is a protected bivalent aromatic structure, tethered to two iminodiacetic acid ester groups N(CHCOOR&#x2033;), or G is a structure selected from the group consisting of or G is a protected functional group, or G is a protected or unprotected organic dye, hapten or a spin label.

Oligonucleotide labeling reactants based on acyclonucleosides and conjugates derived thereof

The invention relates to a novel labeling reactant of formula (I) suitable for labeling an oligonucleotidewherein:R is a temporary protecting group. A is either a phosphorylating moiety or a solid support tethered to a bridge point Z via a linker arm E. E' is a linker arm between G and Z. G is a bivalent aromatic structure, tethered to two iminodiacetic acid ester groups N(COOR&#x2032;&#x2033;)or G is a structure selected from a group consisting ofG is a protected functional group. The invention further concerns a method for direct attachment of a conjugate group to an oligonucleotide structure enabling the attachment of a desired number of these groups during chain assembly. The method comprises a Mitsonobu alkylation.

Oligonucleotide labeling reactants and their use

The invention relates to a novel labeling reactant, suitable for labeling of a biospecific binding reactant using solid-phase synthesis. The invention further concerns new labeling methods. The novel labeling reactant has the formula (I) ##STR1## wherein -A- is a bivalent aromatic structure capable of absorbing light or energy and transferring the excitation energy to a lanthanide ion after the product made by the said solid-phase synthesis has been released from the used solid support, deprotected and converted to a lanthanide chelate;

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Raisio, Finland

Jari Hovinen