Basf Se Corporation

Jared Dennis

Xuqiu Tan

Bin Zhang

Kenneth Barrett

Jochen Kutscher

Adrienne Huston Davenport

Kristine Chang

Tong Li

Hugo D Urbina

Dongmei Ren

Corey Dodge

Robin Cai

Disclosed herein is an improved Gene Site Saturation Mutagenesis (GSSM) method for producing a plurality of modified polynucleotides and/or polypeptides, creating specific changes to a gene, and reassembling mutations or changes at one or more sites.

Gene site saturation mutagenesis (GSSM) method

Disclosed herein are polypeptides having nuclease activity. Some of the polypeptides having nuclease activity were generated by an improved gene site mutagenesis ('GSSM') method or the tailored multi-site combinatorial assembly ('TMCA') method. Also disclosed are compositions and kits comprising the polypeptides having nuclease activity, and methods for making and using these polypeptides, compositions and kits.

Nucleases and methods for making and using them

Variant polypeptides having beta-amylase activity and methods of making and using the enzymes in baking, detergents, personal care products, in the processing of textiles, in pulp and paper processing, in the production of ethanol, lignocellulosic ethanol, or syrups; as viscosity breakers in oilfield and mining industries.

Beta-amylase enzymes

Growing community of inventors

San Diego, CA, United States of America

Jared Dennis