Richter Gedeon Vegyeszeti Gyar Rt

Other

Labor Muszeripari Muvek

Richter Gedeon Vegyeszeti Gmbh

Janos Nagy

Jozsef Knoll

Huba Kalasz

Berta Knoll

Erno Tyihak

Emil Mincsovics

Gyula Horvath

Sandor Zoltan

Peter Tetenyi

Janos Kiss

Laszlo Kozma

Janos Borsos

Istvan Lak

Sandor Dios

Ferenc Kormendi

Sandor Harmath

The selective appetite-regulating substances satietin and satietin-D had up to the present been prepared from human or animal blood serum or plasma by ultrafiltration, gel chromatography, treatment with trichloroacetic acid, affinity chromatography and optionally treatment with a proteolytic enzyme. According to the invention, satietin and satietin-D are obtained in a purified form by using immunoabsorption. Being glycoproteins, both satietin and satietin-D form antibodies (antisatietin and antisatietin-D, respectively) in the living organism and can be isolated from the blood and bound to a gel column. For the preparation of satietin and satietin-D, respectively, the human or animal blood plasma or serum is subjected to ultrafiltration and the ultrafiltrate, containing in addition to satietin and satietin-D other constituents with a molecular weight below 50000 daltons, is contacted with the gel column. The antibody bound to the gel column forms with satietin or satietin-D, respectively, an immunocomplex remaining on the column while the other constituents of the ultrafiltrate can be eluted by a buffer. Then, the immunocomplex is cleaved, whereby satietin or satietin-D, respectively, can be eluted in a purified form from the column. The column containing the antibody may repeatedly be used.

Purified appetite-regulating substances of biological orgin, their

Chromatographic sheet of layer for pressurized layer chromatographic

The invention involves isolating a food intake suppressant by subjecting blood serum to ultrafiltration transmitting up to a molecular weight of 30,000 daltons, partially evaporating the filtrate, removing the insoluble part from the concentrate obtained, adding trichloroacetic acid up to a concentration of 5 to 25 weight/volume percent to the liquid phase at a temperature of 0.degree. to 10.degree. C., removing the proteins precipitated, subjecting the obtained solution to chromatography on a gel with a void volume below a molecular weight of 4000 daltons, eluting with a solution of pH 6.0 to 7.0, concentrating the biologically active fractions, chromatographing again on a gel with a void volume below a molecular weight of 4000 daltons, fractionating by elution with water, lyophilizing the active fractions, dissolving the lyophilized fractions in a buffer of pH 8.1 to 8.2, adding trypsin and chymotrypsin to the solution in a substantially identical amount of 0.01 to 0.2 by weight as calculated for the total weight of the lyophilized product, subjecting the mixture to digestion at a temperature of 36.degree. C. to 39.degree. C., preferably with occasionally shaking from 20 to 30 hours, adding after the first 5 hours trypsin and chymotrypsin in a substantially half amount as calculated for the original enzyme quantity and continuing the digestion, then adding trichloroacetic acid to the reaction mixture up to a concentration of 4 to 6 weight/volume percent at a temperature between 0.degree. and 10.degree. C., keeping the mixture at a temperature between -15.degree. C. and 5.degree. C. for 1 to 24 hours, removing the insoluble part, subjecting the reaction mixture to chromatography on a gel with a void volume below a molecular weight of 4000 daltons, fractionating by elution with water and lyophilizing the biologically active fractions.

Process for the preparation of a new-type active substance selectively

A process is disclosed for the preparation of an endogenous anorexogenic substance obtained from blood serum. The anorexogenic substance itself is also described having a specific activity of 100 units/mg.

Process for the preparation of a selective anorexogenic substance

A linear overpressured thin layer chromatographic apparatus comprises a base plate 3 supporting a sorbent layer 1 over which a membrane 4 is filled. The membrane 4 is externally pressurized by a fluid medium 10. A transparent cover plate 5 is fitted over the membrane 4, and there are sealed inlets 6, 7 passing through the cover plate 5 for the introduction of the mobile phase and the samples.

Linear overpressured thin-layer chromatographic apparatus

Described is the method of preparing satietin, a peptide separated from plasma or serum and possessing the effect of inhibiting food intake. Also described is the product satietin.

Process for the production of an andrexogenic substance having specific

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Szentendre, Hungary

Janos Nagy