Abbott Laboratories Corporation

Janis A Martinus

Cynthia M Molina

Kenward S Vaughan

Catherine M Smith

Paul J Brynes

Paul J Byrnes

This disclosure relates to a method and reagents for determining amphetamine and methamphetamine in a biological fluid such as urine. In particular, this disclosure relates to a fluorescence polarization immunoassay procedure for determining the presence of amphetamine and methamphetamine in a single assay and to a novel class of tracer compounds employed as reagents in such procedures. The procedure described includes pretreatment of the biological sample to eliminate cross-reactants such as .beta.-hydroxyphenethylamine by preincubating the sample solely with an aqueous periodate solution having a pH from about 4.0 to about 7.5 without adjustment to an alkaline pH.

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Wildwood, IL, United States of America

Janis A Martinus