Mammoth Biosciences, Inc.

Janice Sha Chen

Lucas Benjamin Harrington

Pedro Patrick Draper Galarza

James Paul Broughton

Jasmeet Singh

Xin Miao

Ashley Tehranchi

Andrew Besancon Lane

Maria-Nefeli Tsaloglou

Clare Louise Fasching

Wiputra Jaya Hartono

Isaac Paterson Witte

Described herein are devices, systems, fluidic devices, kits, and methods for detection of target nucleic acids associated with diseases, cancers, genetic disorders, a genotype, a phenotype, or ancestral origin. The devices, systems, fluidic devices, kits, and methods may comprise reagents of a guide nucleic acid targeting a target nucleic acid, a programmable nuclease, and a single stranded detector nucleic acid with a detection moiety. The target nucleic acid of interest may be indicative of a disease, and the disease may be communicable diseases, or of a cancer or genetic disorder. The target nucleic acid of interest may be indicative of a genotype, a phenotype, or ancestral origin.

Programmable nuclease compositions and methods of use thereof

Disclosed herein are compositions, kits, and methods related to improved Cas activity. Through compositions and kits disclosed herein and practice of methods disclosed herein, one attains improved Cas activity such as Cas12 activity relative to Cas proteins in the art such as LbCas12. Further described herein are methods to detect target nucleic acid using a programmable nuclease system. Often, the target nucleic acids are present in at low frequency in the sample. Provided herein are methods for enriching these target nucleic acids for detection. Also described herein are methods to insert a PAM sequence into a target sequence of interest for use in a detection comprising a programmable nuclease.

Programmable nuclease improvements and compositions and methods for nucleic acid amplification and detection

Growing community of inventors

San Francisco, CA, United States of America

Janice Sha Chen