Becton Dickinson and Company Limited

Flowjo, LLC

Janice H Lai

Stephen P A Fodor

Glenn K Fu

David Stern

Christina Fan

Eleen Shum

Christina Chang

Katherine Lazaruk

James Ghadiali

Dennis E Prosen

Elisabeth Marie Walczak

Geoff Facer

Devon Joseph Jensen

David Rosenfeld

Miguel Velazquez-Palafox

Ian Taylor

Devon Jensen

Ari Chaney

Xiaohua Chen Huang

Kimberly R Metzler

Sixing Li

Methods and systems for a deep-learning platform for sorting cell populations. An example method includes executing a software-platform associated with analyzing received flow cytometry data obtained via an acquisition device in communication with the computing system, and the software-platform sorting cell populations indicated in the flow cytometry data. User input is received indicating selection of a deep-learning module, the deep-learning module being obtained via a network to supplement the software-platform. The flow cytometry data is analyzed and a machine learning model is selected which was trained based on similar phenotype information as indicated in the flow cytometry data. The machine learning model is applied based on the flow cytometry data, the information being normalized based on the UMI counts associated with the flow cytometry data. A graphical representation of cell populations indicated in the flow cytometry data is presented, the graphical representation sorting the cell populations according to phenotype information.

Deep learning particle classification platform

Disclosed herein include systems, methods, compositions, and kits for determining protein expression and gene expression simultaneously and for sample indexing. In some embodiments, an oligonucleotide associated with a cellular component-binding reagent (e.g., an antibody) comprises one or more of a unique molecular label sequence, a primer adapter, antibody-specific barcode sequence, an alignment sequence, and/or a poly(A) sequence. In some embodiments, the oligonucleotide is associated with the cellular component-binding reagent via a linker (e.g., 5AmMC12).

Oligonucleotides associated with antibodies

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Mountain View, CA, United States of America

Janice H Lai