Roche Vitamins Inc.

Omnigene, Inc.

Biotechnica International, Inc.

Basf Aktiengesellschaft

Basf Se Corporation

Hoffmann-la Roche Inc.

Dsm IP Assets B.v.

Evonik Degussa Gmbh

Ptt Global Chemical Public Company Limited

Dsm Nutritional Products Inc.

Myriant Corporation

Janice G Pero

Alan Sloma

R Rogers Yocum

Theron Hermann

John B Perkins

Thomas A Patterson

Cathy F Rudolph

Gerald A Rufo, Jr

Thomas John Erdenberger

Randolph T Hatch

Barbara J Sullivan

Stanley Grant Bower

Sudhanshu Vijay Dole

Nancy M Hannett

R Roger Yocum

Oskar Zelder

Hartwig Schröder

Stefan Haefner

Corinna Klopprogge

Kai-Uwe Baldenius

Andrea Herold

Christoph Wittmann

Phillips W Robbins

Christine Beck

Wei Gong

Elmar Heinzle

Tammy Grabar

Mark K Williams

Ryan Sillers

Andrew Christopher Collard

Jens Kroemer

Karen M Overbye

Theron Herman

Michael A Stephens

Diane L Stassi

Phillips Robbins

Sonia Guterman

Rogers R Yocum

Scott W Van Arsdell

Rosalind C Lee

M A Stephens

Sudhanshu Dole

Michelle Spencer

Meghal Gandhi

Hartwig SCHRÖDER

Mark Williams

This invention relates to the biosynthesis of organic acids in genetically modified microorganisms. More specifically, this invention provides genetically modified microorganisms that are particularly tolerant to organic acids at low pH and are capable of producing organic acids by fermentation at low pH.

Production of organic acids by fermentation of genetically engineered yeast cells at low pH

This present invention relates to production of chemicals from microorganisms that have been genetically engineered and metabolically evolved. Improvements in chemical production have been established, and particular mutations that lead to those improvements have been identified. Specific examples are given in the identification of mutations that occurred during the metabolic evolution of a bacterial strain genetically engineered to produce succinic acid. This present invention also provides a method for evaluating the industrial applicability of mutations that were selected during the metabolic evolution for increased succinic acid production. This present invention further provides microorganisms engineered to have mutations that are selected during metabolic evolution and contribute to improved production of succinic acid, other organic acids and other chemicals of commercial interest.

Engineering microbes for efficient production of chemicals

The present invention features methods of producing panto-compounds (e.g., pantothenate) using microorganisms in which the pantothenate biosynthetic pathway and/or the isoleucine-valine biosynthetic pathway and/or the coenzymeA biosynthetic pathway has been manipulated. Methods featuring ketopantoate reductase overexpressing microorganisms as well as aspartate &#x3b1;-decarboxylase overexpressing microorganisms are provided. Methods of producing panto-compounds in a precursor-independent manner and in high yield are described. Recombinant microorganisms, vectors, isolated nucleic acid molecules, genes and gene products useful in practicing the above methodologies are also provided. The present invention also features a previously unidentified microbial pantothenate kinase gene, coaX, as well as methods of producing panto-compounds utilizing microorganisms having modified pantothenate kinase activity. Recombinant microorganisms, vectors, isolated coaX nucleic acid molecules and purified CoaX proteins are featured. Also featured are methods for identifying pantothenate kinase modulators utilizing the recombinant microorganisms and/or purified CoaX proteins of the present invention.

Methods and microorganisms for production of panto-compounds

The present invention relates to microorganisms and processes for the efficient preparation of L-amino acids such as L-methionine. In particular, the present invention relates to microorganisms and processes in which the formation and/or accumulation of homolanthionine in the methionine pathway is reduced and/or prevented.

Microorganism and process for the preparation of L-methionine

The present invention features improved methods for the enhanced production of pantoate and pantothenate utilizing microorganisms having modified pantothenate biosynthetic enzyme activities and having modified methylenetetrahydrofolate (MTF) biosynthetic enzyme activities. In particular, the invention features methods for enhancing production of desired products by increasing levels of a key intermediate, ketopantoate, by increasing enzymes or substrates that contribute directly or indirectly to its synthesis. Recombinant microorganisms and conditions for culturing same are also are featured. Also featured are compositions produced by such microorganisms.

Microorganisms and processes for enhanced production of pantothenate

The present invention features improved methods for the enhanced production of pantoate and pantothenate utilizing microorganisms having modified pantothenate biosynthetic enzyme activities and having modified methylenetetrahydrofolate (MTF) biosynthetic enzyme activities. In particular, the invention features methods for enhancing production of desired products by increasing levels of a key intermediate, ketopantoate by enzymes that contribute to its synthesis. Recombinant microorganisms and conditions for culturing same are also are featured. Also featured are compositions produced by such microorganisms.

The present invention features improved methods for producing pantoate and pantothenate utilizing microorganisms having modified pantothenate biosynthetic enzyme activities. In particular, the invention features methods for reducing byproduct formation and increasing yields and purity of desired product. Recombinant microorganisms and conditions for culturing same are also are featured. Also featured are compositions produced by such microorganisms.

Processes for enhanced production of pantothenate

Vectors and recombinant bacteria for overproducing riboflavin, in which nucleic acid overproducing riboflavin biosynthetic proteins is introduced in the chromosome of the host organism, e.g. at multiple sites and in multiple copies per site. A rib operon having at least five genes is used to make such recombinant bacteria.

Bacterial strains which overproduce riboflavin and methods of use thereof

The invention generally provides the genes of the biotin biosynthetic operon of, and closely related species thereof, and well as constructions useful for high level production of biotin.

Biotin biosynthesis in bacillus subtilis

A method is disclosed for the increased production of biotin and the biotin precursor dethiobiotin using a bacterium that produces a lysine-utilizing DAPA aminotransferase. This method involves the use of a bacterium that is either grown in the presence of lysine or deregulated for lysine biosynthesis.

Overcoming DAPA aminotransferase bottlenecks in biotin vitamers biosynthesis

Nutrient medium for bacterial strains which overproduce riboflavin

The invention generally provides the genes of the biotin biosynthetic operon of,, and closely related species thereof, and well as constructions useful for high level production of biotin.

The invention generally provides the genes of the biotin biosynthetic operon of Bacillus subtilis, and closely related species thereof, and well as constructions useful for high level production of biotin.

Biotin biosynthesis in Bacillus subtilis

Bacterial strains which overproduce riboflavin

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Lexington, MA, United States of America

Janice G Pero