Chiron Corporation

Bayer Corporation

The Director General of the National Institute of Health of Japan

Janice A Kolberg

Michael S Urdea

Bruce D Irvine

Tai-An Cha

Michael Houghton

Amy J Weiner

Jang Hyun Han

Eileen Beall

Joyce A Running

Qui-Lim Choo

Brian David Warner

George Kuo

Thomas Horn

Tatsuo Miyamura

Izumi Saito

Jennifer M Clyne

Ray Sanchez-Pescador

P Scott Eastman

Lu-Ping Shen

Novel DNA probe sequences for detection of HIV in a sample in a solution phase sandwich hybridization assay are described. Amplified nucleic acid hybridization assays using the probes are exemplified.

HIV probes for use in solution phase sandwich hybridization assays

The present application features nucleic acid, peptide and antibody compositions relating to genotypes of hepatitis C virus and methods of using such compositions for diagnostic and therapeutic purposes.

HCV genomic sequences for diagnostics and therapeutics

Synthetic oligonucleotides which are useful as probes in a solution phase sandwich hybridization assay for the detection of HTLV-1 are described. The oligonucleotides have a first segment comprising a nucleotide sequence substantially complementary to a segment of HTLV-1 nucleic acid, and a second segment comprising a nucleotide sequence which is not complementary to HTLV-1 nucleic acid but is substantially complementary to an oligonucleotide unit of a nucleic acid multimer or to an oligonucleotide bound to a solid phase. Amplified nucleic acid hybridization assays using the probes are exemplified.

HTLV-1 probes for use in solution phase sandwich hybridization assays

This invention relates to a method of measuring the relative populations of first and second variants of a target nucleotide sequence of a target genome in a sample utilizing an amplification step, followed by probing with first and second variant probes and a control probe. Specific embodiments include an assay to monitor the conversion of codon 215 for HIV-1 reverse transcriptase from wild type to mutant form.

Differential hybridization for relative quantification of variant populations

Two new isolates of the Hepatitis C virus (HCV), J1 and J7, are disclosed. These new isolates comprise nucleotide and amino acid sequences which are distinct from the prototype HCV isolate, HCV1. Thus, J1 and J7 provide new polynucleotides and polypeptides for use, inter alia, in diagnostics, recombinant protein production and vaccine development.

Hepatitis C virus isolate polypeptides

Differential hybridization for relative quantification of variant

Novel DNA probe sequences for detection of HBV in a sample in a solution phase sandwich hybridization assay are described. Amplified nucleic acid hybridization assays using the probes are exemplified.

HBV capture and amplifiers probes for use in solution phase sandwich

A new virus, Hepatitis C virus (HCV), which has proven to be the major etiologic agent of blood-borne NANBH, was discovered by Applicant. Reagents for isolating, amplifying, and detecting HCV polynucleotides are provided. These reagents are oligomers comprised of polynucleotide sequences which are capable of forming hybrid structures with HCV target polynucleotide sequences.

NANBV diagnostics: polynucleotides useful for screening for hepatitis C

A new virus, Hepatitis C virus (HCV), which has proven to be the major etiologic agent of blood-borne NANBH, was discovered by Applicant. Reagents for isolating, amplifying, and detecting HCV polynucleotides are provided. These reagents are oligomers containing polynucleotide sequences which are capable of forming hybrid structures with HCV target polynucleotide sequences.

Methods for detecting Hepatitis C virus using polynucleotides specific

Novel DNA probe sequences for detection of HAV in a sample in a solution phase sandwich hybridization assay are described. Amplified nucleic acid hybridization assays using the probes are exemplified.

HAV probes for use in solution phase sandwich hybridization and assays

Linear or branched oligonucleotide multimers useful as amplifiers in biochemical assays which comprise (1) at least one first single-stranded oligonucleotide unit that is complementary to a single-stranded oligonucleotide sequence of interest, and (2) a multiplicity of second single-stranded oligonucleotide units that are complementary to a single-stranded labeled oligonucleotide. Amplified sandwich nucleic acid hybridizations and immunoassays using the multimers are exemplified.

Nucleic acid hybridization assay for hepatitis B virus DNA

Novel DNA probe sequences for detection of CMV in a sample in a solution phase sandwich hybridization assay are described. Amplified nucleic acid hybridization assays using the probes are exemplified.

Growing community of inventors

Hercules, CA, United States of America

Janice A Kolberg