University of Georgia Research Foundation, Inc.

Ut-battelle, Inc.

University of Tennessee Research Foundation

Janet Westpheling

Julie A Burke

Joel Andrew Farkas

Daehwan Chung

Adam Michael Guss

Michael W W Adams

Lauren A Riley

Jennifer Huddleston

Minseok Cha

Gina L Lipscomb

This disclosure describes recombinantmicrobes designed to include increased consumption of acetate, increased carbon flux to butyryl-CoA and/or hexanoyl-CoA, increased production of butyrate and/or hexanoate, or a combination thereof, than a comparable control. This disclosure also describes methods that generally include growing such recombinant microbes under conditions effective for the recombinant microbes to consume greater amounts of acetate, produce increased amounts of butyryl-CoA and/or hexanoyl-CoA, produce increased amounts of butyrate and/or hexanoate, or a combination thereof.

Microbial approach for the production of long chain compounds

This disclosure describes recombinantmicrobes designed to produce greater amounts of acetate, H, and/or ethanol than a comparable wild type control. this disclosure also describes methods that generally include growing such recombinant microbes under conditions effective for the recombinant microbes to produce acetate, H, and/or ethanol.

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The present invention relates to the discovery of a novel restriction/modification system in. The discovered restriction enzyme is a HaeIII-like restriction enzyme that possesses a thermophilic activity profile. The restriction/modification system also includes a methyltransferase, M.CbeI, that methylates at least one cytosine residue in the CbeI recognition sequence to mC. Thus, the invention provides, in various aspects, isolated CbeI or M.CbeI polypeptides, or biologically active fragments thereof; isolated polynucleotides that encode the CbeI or M.CbeI polypeptides or biologically active fragments thereof, including expression vectors that include such polynucleotide sequences; methods of digesting DNA using a CbeI polypeptide; methods of treating a DNA molecule using a M.CbeI polypeptide; and methods of transforming acell.

Restriction/modification polypeptides, polynucleotides, and methods

Provided herein are methods for transforming awith a polynucleotide. In one embodiment, the method includes contacting awith a polynucleotide under conditions suitable for uptake of the polynucleotide by the, and identifying transformants at a frequency of, for instance, at least 10transformants per microgram DNA. Also provided are isolatedhaving the characteristics ofCOM1, and plasmids that include an origin of replication that functions in a. The plasmid is stable in a recipientwithout selection for more than 100 generations and is structurally unchanged after replication infor more than 100 generations.

The present invention is directed to isolated transducing phages, methods of isolating transducing phages, and methods of using transducing phages including, for instance, transferring at least one nucleic acid fragment from a donor microbe to a recipient microbe, and producing a secondary metabolite from a microbe. The transducing phages typically have a broad host range, and transduce microbes in the Order Actinomycetales, in particular in the Family Streptomycetaceae, including , and . The transducing phages can be specialized transducing phages or generalized transducing phages.

Transducing phages

The present invention is directed to isolated transducing phages, methods of isolating transducing phages, and methods of using transducing phages including, for instance, transferring at least one nucleic acid fragment from a donor microbe to a recipient microbe, and producing a secondary metabolite from a microbe. The transducing phages typically have a broad host range, and transduce microbes in the Order Actinomycetales, in particular in the Family Streptomycetaceae, including,and,The transducing phages can be specialized transducing phages or generalized transducing phages.

Growing community of inventors

Bogart, GA, United States of America

Janet Westpheling