Danstar Ferment Ag

Lallemand Hungary Liquidity Management LLC

Lallemand USA, Inc.

Janet Fisher

Aaron Argyros

Michelle Oeser

Charles F Rice

Brooks Henningsen

Trisha Barrett

Allan C Froehlich

Erin Wiswall

Ryan Skinner

Kevin S Wenger

Rintze M Zelle

J Kevin Kraus

Johannes Van Eijk

The present disclosure concerns the use of specific genetic modification(s) for improving sulfite tolerance in recombinant yeast host cells. The genetic modification(s) is (are) designed to allow the expression of an heterologous transcription factor favoring the expression of a SSU1 polypeptide and/or the expression of an heterologous SSU1 polypeptide in the recombinant yeast host cell(s).

Sulfite tolerance in recombinant yeast host cells

The present disclosure concerns yeast host cell especially suited for the expression of heterologous proteins, such as heterologous enzymes. The yeast host cell of the present disclosure exhibits an alteration in the cAMP signaling pathway which allows achieving increased heterologous protein yield and associated biological activity. The yeast host cell can also exhibit polyploidy.

Optimization of yeast host cells for the production of heterologous proteins

The present disclosure concerns recombinant yeast host cells expressing cell-associated heterologous food and/or feed enzymes which are expressed during the propagation phase of the recombinant yeast hosts cells. The recombinant yeast host cells can be used in a subsequent production process to make food and/or feed products, for example, baked products.

Cell-associated heterologous food and/or feed enzymes

The present disclosure relates to the modulation in the RAS/cAMP/PKA signaling pathway for maintaining the propagation efficiency and increasing fermentation efficiency of yeast cells. The present disclosure provides yeast cells having or engineered to exhibit a modulation in signaling in a RAS/cAMP/PKA pathway, depending on conditions. For example the yeast cells can be selected or genetically modified to express a mutated Ras1 protein, a mutated Ras2 protein, a mutated Ira1 protein and/or a mutated Ira2 protein, optionally in combination with specific promoters. Also provided herewith are methods for propagating the yeast cells as well as using the yeast cells to generate a fermented product (such as ethanol).

Regulation of the RAS/cAMP/PKA signaling pathway in yeasts for obtaining a fermentation product during fermentation

Growing community of inventors

Ossipee, NH, United States of America

Janet Fisher