Dh Technologies Development Pte. Ltd.

Jane Luo

Chitra Kumari Ratnayake

Brian D Peterson

Sunil S Deliwala

Craig A Hudson

Handy Yowanto

Peter S Kotowski

Tom Tran

Anna X Lou

Dale Alan Owens

Jean-Marc Rene Lucien Busnel

James H Reyes

Elliot B Jones

Clive Cheung

A CE based method and kit for the determination of the size and purity of an AAV genome which relies on Capillary Electrophoresis-Laser Induced Fluorescence (CE-LIF) analysis. These methods and kits are capable of detecting intact and partial genomes in a virus vectors such as adeno-associated viruses as well as remove small size impurities. In one example, the method can include creating a nucleic acid ladder using CE-LIF, releasing the genome from within an adeno-associated virus, purifying said genome and analyzing said genome using CE-LIF and comparing the results of the analysis of the genome to the nucleic acid ladder to determine a size of nucleic acids in the genome.

Genome integrity analysis of adeno-associated virus vectors

Aspects of the innovations presented herein relate to improved systems that in some embodiments perform capillary electrophoresis (CE) and CE in conjunction with electrospray ionization (ESI) as an input to a mass spectrometry system (MS). Some embodiments use a high voltage isolated CE power supply that is configured to float on the high voltage output of an ESI-MS power supply, with a protective resistance in the ESI-MS path, as well as DC/DC converter isolation and communication system isolation for the isolated CE power supply. Some embodiments additionally use a cartridge assembly integrating separation and conductive fluid capillaries with fluid cooling and protective retractable housings for the capillary end portions and for the ESI output. The protective housing may further be used with an adapter for interfacing with different MS systems.

Capillary electrophoresis-electrospray ionization-mass spectrometry system

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Irvine, CA, United States of America

Jane Luo