Pfenex Inc.

Exxonmobil Research and Engineering Company

Cp Kelco U.s., Inc.

Fundacion Instituto Leloir

Dow Global Technolgoies LLC

Jane C Schneider

Thomas Martin Ramseier

Diane M Retallack

Russell J Coleman

Charles D Hershberger

Yamini N Patel

Charles H Squires

Maria Veronica Ielmini

Luis Ielpi

Eric R Moellering

Bo Liu

Soyan Leung Lieberman

Thomas M Ramseier

John Verruto

Rekha Seshadri

Sol M Resnick

Lawrence C Chew

Stacey Lynn Lee

Erik Holtzapple

Gena Roy

Amanda Skaggs

Anne Kathryn Badgley

Sol Resnick

Russell John Coleman

Charles Douglas Hershberger

Diane Retallack

Stacey L Lee

Luis Lelpi

Maria Veronica Lelmini

The present invention provides an array for rapidly identifying a host cell population capable of producing a heterologous recombinant protein with improved yield and/or quality. The array comprises one or more host cell populations that have been genetically modified to increase the expression of one or more target genes involved in protein production, decrease the expression of one or more target genes involved in protein degradation, or both. One or more of the strains in the array may express the heterologous recombinant protein of interest in a periplasm compartment or may secrete the heterologous recombinant protein extracellularly through an outer cell wall. The strain arrays are useful for screening for improved expression of any protein of interest including therapeutic proteins, hormones, growth factors, extracellular receptors or ligands, proteases, kinases, blood proteins, chemokines, cytokines, antibodies and the like.

Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins

The present invention provides novel promoter and terminator sequences for use in heterologous gene expression in eukaryotic cells, such as algal cells. The invention further provides expression cassettes comprising a promoter, as described herein, operably linked to a heterologous gene. The invention further provides expression vectors and host eukaryotic cells, such as algal cells, for expressing a protein encoded by the heterologous gene; and methods for identifying promoters.

Promoters and terminators for use in eukaryotic cells

The present invention provides an array for rapidly identifying a host cell population capable of producing a heterologous protein with improved yield and/or quality. The array comprises one or more host cell populations that have been genetically modified to increase the expression of one or more target genes involved in protein production, decrease the expression of one or more target genes involved in protein degradation, or both. One or more of the strains in the array may express the heterologous protein of interest in a periplasm compartment or may secrete the heterologous protein extracellularly through an outer cell wall. The strain arrays are useful for screening for improved expression of any protein of interest including therapeutic proteins, hormones, growth factors, extracellular receptors or ligands, proteases, kinases, blood proteins, chemokines, cytokines, antibodies and the like.

The present invention provides diacylglycerol acyltransferase (DGAT) genes, including genes encoding localization peptides. The present invention also provides recombinant cells, such as algae, transformed with DGAT genes and methods of using such recombinant cells to produce triglyceride.

DGAT genes and methods of use for triglyceride production in recombinant microorganisms

The present invention provides novel promoter and terminator sequences for use in gene expression in eukaryotic cells, such as algal cells. The invention further provides expression cassettes comprising a promoter, as described herein, operably linked to a gene. The invention further provides expression vectors and host eukaryotic cells, such as algal cells, for expressing a protein encoded by the gene; and methods for stably transforming eukaryotic algae such aswith transgenes.

Tetraselmis promoters and terminators for use in eukaryotic cells

The present invention provides an improved expression system for the production of recombinant polypeptides utilizing auxotrophic selectable markers. In addition, the present invention provides improved recombinant protein production in host cells through the improved regulation of expression.

Protein expression systems

Increasing the molecular length of xanthan polymer makes a higher viscosity xanthan composition. Xanthan with higher specific viscosity characteristics provides more viscosity at equivalent concentration in food, industrial and oilfield applications. Methods for increasing the viscosity of xanthan include inducing particular key genes and increasing copy number of particular key genes.

High viscosity xanthan polymer preparations

Improved methods and prokaryotic expression systems for producing recombinant proteins using Sec-system secretion signal peptides are described usingSec secretion systems. Specific novel Sec-system secretion signal peptides are described, as are fusion proteins and coding sequences for improved secretion of recombinant proteins and peptides.

Expression systems with sec-system secretion

Compositions and methods for improving expression and/or secretion of protein or polypeptide of interest in a host cell are provided. Compositions comprising a coding sequence for a bacterial secretion signal peptide are provided. The coding sequences can be used in vector constructs or expression systems for transformation and expression of a protein or polypeptide of interest in a host cell. The compositions of the invention are useful for increasing accumulation of properly processed proteins in the periplasmic space of a host cell, or for increasing secretion of properly processed proteins from the host cell. In particular, isolated secretion signal peptide-encoding nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the nucleic acid molecules are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequences shown in SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, and 24, and the nucleotide sequences set forth in SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, and 23, as well as variants and fragments thereof.

Bacterial leader sequences for increased expression

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San Diego, CA, United States of America

Jane C Schneider