University of California

Wisconsin Alumni Research Foundation

Other

University of Connecticut

Imperial College Innovations Limited

Jane C Burns

Robert D Bremel

Theodore Friedmann

Jiing-Kuan Yee

Anthony W S Chan

Thomas T Chen

Myrsini Kaforou

Michael Levin

Anthony W Chan

Clive Hoggart

Victoria Wright

Jethro Herberg

Anthony Ws Chan

A method of identifying a subject having Kawasaki disease (KD), which includes discriminating the subject from a subject having another condition, for example other infectious and inflammatory conditions, such as those that present similar symptoms to KD. Also provided is a minimal gene signature employed in the method, as well as primers, probes and gene chips for use in the method.

Method of identifying a subject having Kawasaki disease

The present invention provides improved methods and compositions for the generation of transgenic non-human animals. The present invention permits the introduction of exogenous nucleic acid sequences into the genome of unfertilized eggs (e.g., pre-maturation oocytes and pre-fertilization oocytes) by microinjection of infectious retrovirus into the perivitelline space of the egg. The methods of the present invention provide an increased efficiency of production of transgenic animals with a reduced rate of generating animals which are mosaic for the presence of the transgene.

Transgenic animals

Methods for creating transgenic animals

A carrier and hitch assembly comprises a rigid, elongated housing having a door covered opening on a front face. The housing is large enough to contain a standard size golf bag and a complete set of clubs. The housing is provided with wheels and a handle so that the housing may be utilized in cart-like fashion. The rear face of the housing is provided with structure for attaching the housing to a hitch assembly for transporting the housing at the rear of an automotive vehicle or golf cart.

Golf bag and cart carrier

A method for introducing foreign nucleic acid sequences into marine mollusks. A pantropic retroviral vector containing a foreign gene sequence is introduced into fertilized mollusk embryos by electroporation. The gene sequence becomes integrated into the host DNA and encodes a functional protein product. This method has implications in the introduction of disease-resistance and growth-accelerating genes into mollusks.

Pantropic retroviral vectors for gene transfer in mollusks

The present application discloses retrovirus-derived vectors in which the retroviral envelope glycoprotein has been replaced by the G glycoprotein of vesicular stomatitis virus, and the use of these vectors in the transfer of exogenous genes into the cells of a wide variety of non-mammalian organisms. Also disclosed is a method for the generation of retroviral vectors in high titers, wherein a recombinant, stable host cell line is provided which harbors the retroviral vector of interest without envelope protein. High-titer retroviral vector production is initiated by introducing nucleic acid encoding a functional membrane-associated protein into the cell line. The vectors disclosed in the present application can be concentrated by ultracentrifugation to titers greater than 10.sup.9 cfu/ml which are especially useful in human gene therapy trials, and can also infect cells, such as hamster and fish cells, that are ordinarily resistant to infection with vectors containing the retroviral envelope protein.

Use of VSV-G pseudotyped vectors for transfer of genes into embryos

Generation, concentration and efficient transfer of VSV-G pseudotyped

Growing community of inventors

La Jolla, CA, United States of America

Jane C Burns