Roche Diagnostics Gmbh

Boston Probes, Inc.

J&ouml;rg Kleiber

Herbert Harttig

Helmut Schmidt

Martin Mennig

Thomas Walter

Gregor Sagner

Christoph Lesniak

Christine Markert-Hahn

Karim Tabiti

Albert Geiger

Michael Riedling

Ane-Ullerup Lester

Henrik &Oslash;rum

The invention concerns a process for preparing biological samples for the subsequent detection of an analyte. In particular, the invention relates to a process for the isolation of a nucleic acid in a sample using a suspension of magnetic glass particles. In addition, kits and apparatuses containing magnetic glass particles for sample preparation are provided.

Method, kit and apparatus comprising magnetic glass particles for the isolation of biomolecules

A method for the detection of point mutation and polymorphisms in nucleic acids or for sequencing of unknown nucleic acids by a simple procedure using arrays uses nucleic acid analoges as sequence discriminators. This procedure simplifies the working mode in complex problematic cases.

Sequence specific detection of nucleic acids using a solid carrier bound with nucleic acid analog probes

The present invention concerns a method and a device which enables an integrated amplification and detection of nucleic acids.

Integrated method and system for amplifying and for detecting nucleic acids

Magnetic glass particles are prepared containing a magnetic core coated with a glass layer having a substantially pore-free glass surface. The particles are used for separating biological material such as nucleic acids. A preferred process of preparing the particles is by forming a mixture of magnetic cores with a sol formed from an alcohol and a metal alkoxide, spray-drying the mixture to coat the cores with a layer of gelled sol, and heating the coated cores to obtain the magnetic glass particles. Preferably, the particles have an average particle size of less than 100 &mgr;m and any pores of the glass surface have a diameter of less than 10 nm. The magnetic core may be a composite material containing a mica core and magnetite particles immobilized on the mica core, and the glass layer may contain boron oxide. Magnetic core materials include magnetite (Fe,O,) and Fe,O,. In using the magnetic glass particles to separate a biological material, the particles are contacted with a fluid containing the biological material such that the biological material binds to the glass surface, and the bound biological material is separated from the fluid such as by using a magnetic field. Before applying a magnetic field, the magnetic particles may sediment when contacted with the biological material.

Particles having a magnetic core and outer glass layer for separating biological material

Growing community of inventors

Penzberg, Germany

Jörg Kleiber

Growing community of inventors

Penzberg, Germany

J&ouml;rg Kleiber

Jörg Kleiber