Calysta, Inc.

Jana Stumpe

Jeremy S Minshull

Joshua A Silverman

Jon Edward Ness

Mark D Welch

Effendi Leonard

Renee M Saville

The present disclosure provides nucleic acids and vectors for use with methanotrophic bacteria. Related host cells and methods for using such nucleic acids and vectors for expressing polypeptides or other genetic manipulation of methanotrophic bacteria are also provided.

Nucleic acids and vectors for regulated target gene expression in methanotrophic bacteria

The present disclosure provides compositions and methods for making and using methanotrophic proline auxotrophs.

Proline auxotrophs

The present disclosure provides nucleic acids and vectors for use with methanotrophic bacteria. Related host cells and methods for using such nucleic acids and vectors for expressing polypeptides or other genetic manipulation of methanotrophic bacteria are also provided. In one aspect, the present disclosure is directed to a non-naturally occurring nucleic acid molecule, comprising (1) a promoter that is functional in a methanotrophic bacterium, and (2) a native or altered methanol dehydrogenase (MDH) ribosomal binding sequence, provided that when the promoter is an MDH gene promoter, the nucleic acid comprises an altered MDH ribosomal binding sequence.

Growing community of inventors

Sunnyvale, CA, United States of America

Jana Stumpe