University of California

Max-planck-gesellschaft Zur F&#xf6;rderung Der Wissenschaften E. V.

Jan Zarzycki

Cheryl A Kerfeld

Krishna K Niyogi

Lennart Schada Von Borzyskowski

Patrick M Shih

Tobias Jürgen Erb

The present invention relates to autotrophic microorganisms with altered photorespiration and improved COfixation as well as a method of producing said autotrophic microorganisms. Particularly, the autotrophic microorganisms show an improved growth rate, productivity and energy conversion efficiency.

Method of producing autotrophic organisms with altered photorespiration and improved COfixation

Methods and compositions to introduce a synthetic pathway based on the 3-hydroxypropionate bicycle into an organism such as the model cyanobacterium,sp. PCC 7942. The heterologously expressed pathway acts as a photorespiratory bypass as well as an additional carbon fixation cycle orthogonal to the endogenous Calvin-Benson cycle. We demonstrate the function of all six introduced enzymes, which not only has implications on increasing net-photosynthetic productivity, but also key enzymes in the pathway are involved in high-value products that are of biotechnological interest, such as 3-hydroxypropionate.

Constructs and systems and methods for engineering a COfixing photorespiratory by-pass pathway

Growing community of inventors

Marburg, Germany

Jan Zarzycki