Carl Zeiss Jena Gmbh

Carl Zeiss Microimaging Gmbh

Jan Thirase

Rolf Wartmann

Bruene Venus

A method by which the illumination radiation to be coupled into the illumination beam path of a microscope can be exactly monitored and/or adjusted. In the method for adjusting a light source for a microscope, an imaging optical reflection unit, instead of the objective, is coupled to the microscope in order to image the light of the light source on a detector in such a way that a monitoring of the focus position and/or the position and/or an adjustment of the laser light source with respect to the focus position and/or the position can be carried out. Provided is an approach for monitoring and adjusting a laser beam to be coupled into the illumination beam path of a microscope particularly for using TIRF effects. It can also be used in principle for other solutions in which the parallelism of illumination beams must be monitored and/or adjusted.

Method for the adjustment of a light source in a microscope

The invention is directed to a stereoscopic microscope objective. A microscope objective of this kind contains five lenses. The first lens, considered from the object side, has a focal length between &#x2212;2.11f and &#x2212;1.20f; f is the total focal length of the microscope objective. This is followed by a second lens with a focal length between 1.30f and 1.45f, a third lens with a focal length between 1.15f and 1.37f, a fourth lens with a focal length between 0.65f and 1.7f, and a fifth lens with a focal length between &#x2212;0.96f and 0.48f. Combined, the amount of the refractive power of the last two lenses is less than 0.31f. In the first lens and fifth lens, at least 80% of the refractive power is concentrated on the image-side surfaces. In the second lens, at least 60% of the refractive power is concentrated on the image-side surface. In the third lens, at least 60% of the refractive power is concentrated on the object-side surface. In the fourth lens, at least 70% of the refractive power is concentrated on the image-side surface. The first lens and the fifth lens are made of a material having an anomalous partial dispersion of a first type. The second lens, third lens and fourth lens are made of a material having an anomalous partial dispersion of a second type.

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Goettingen, Germany

Jan Thirase