Arcis Biotechnology Holdings Limited

Arcis Biotechnology Limited

Jan Rogers

Carlos Toro Rueda

Paul Reeves

Benjamin David Cobb

George Victor Garner

Victoria Taylor

A method of detection and identification of one or more microorganism/s in a biological sample comprising the following steps:

Methods and kits

A method of determining of the presence or absence of a target RNA component in a sample, the method comprising steps of: (a) contacting the sample with a composition comprising (i) a quaternary ammonium compound or a precursor thereof, which should extract/release the nucleic acid from the sample; (b) optionally storing and/or transporting the composition obtained in step (a); (c) contacting the composition obtained in step (a) with a composition comprising a proteinaceous washing agent, e.g. BSA or casein for stabilizing the RNA for transport and/or storage; and (d) using the composition obtained in step (c) in an amplification method, e.g. RT-PCR.

Method and composition using a quaternary ammonium compound

A method of extracting DNA and/or RNA from a cell or capsid, the method comprising steps of: (a) contacting a composition comprising the cell or capsid with a composition comprising (ii) a quaternary ammonium compound or a precursor thereof; and (b) contacting the composition obtained in step (a) with a composition comprising a proteinaceous washing agent.

Method and composition

A method of detection and identification of one or more microorganism/s in a biological sample comprising the following steps: (a) extracting DNA from the microorganism/s; and (b) amplifying the extracted DNA and indicating the level of extracted DNA in a quantitative PCR; wherein the quantitative PCR is performed using the primer pair of SEQ ID NO. 1 and 2 together with the probe of SEQ ID NO. 7, and/or the primer pair of SEQ ID NO. 3 and 4 together with the probe of SEQ ID NO. 8.

A method (e.g. high resolution melting analysis) of determining of the presence or absence of a target nucleic acid in a sample, e.g. a biological sample, the method comprising steps of:&#x22c5;(a) contacting the sample with a composition comprising&#x22c5;(i) a quaternary ammonium compound, e.g. 3-(trimethoxysilyl) propyl-dimethyl octadecyl ammonium chloride (3-TPAC) or a precursor thereof, in order to release the nucleic acids from said sample; and&#x22c5;(b) contacting the sample with a probe which is capable of interacting with the nucleic acid sequence; and&#x22c5;(c) observing and/or measuring a property of the sample, e.g. fluorescence from a dsDNA binding dye.

A method of inducing competence in cells, the method comprising contacting the cell with a composition comprising a quaternary ammonium compound including a silicon-containing functional group and a hydrocarbyl-saccharide compound.

Permeability and transformation of cells

There is described a method of extracting DNA and/or RNA from a cell or capsid, the method comprising contacting the cell or capsid with a composition comprising a quaternary ammonium compound including a silicon-containing functional group. The quaternary ammonium compound may be of general formula (I) or a derivative salt thereof wherein L is a linking group; each of R, R, R, R, Rand Ris independently selected from H or an optionally substituted alkyl, alkenyl, aryl or alkoxy group; and n is 0 or 1. A PCR promoting agent may be provided. The method may be one of 1 detecting and/or diagnosing a disease or medical condition.

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Chester, United Kingdom

Jan Rogers