Pharmacia Aktiebolag

Pharmacia Diagnostics Ab

Pharmacia Fine Chemicals Ab

Pharmacia Ab

Wallac Oy

Telia Ab

Pharmacia & Upjohn Diagnostic Ab

Jan P Carlsson

Rolf E Axen

Hakan N Drevin

Goran E Lindgren

Sven O Oscarsson

Marek Kwiatkowski

Ilkka Hemmila

Timo Lovgren

Hakan Drevin

Maria Lönnberg

Maria L&ouml;nnberg

Per Hoglund

Jesper Skagerberg

Anna T Martin

Per H Ponterius

The invention relates to an analytical chromatographic method which comprises the steps of: The invention also relates to a chromatographic device comprising a membrane type flow matrix attached to a liquid-impervious backing, which membrane permits a capillary force assisted lateral fluid flow therethrough and at least a part of which is modified to support ion-exchange functions.

Analytical method and advice

The invention relates to a method for determining an analyte by means of binding reactions, which method comprises: i) applying the sample to an application zone for sample (ASZ) on a flow matrix in which transport of components present in the sample can take place (transport flow), the flow matrix further exhibiting: a) optionally an application zone (AR*Z) for a binding reactant (Reactant*&equals;R*) which is analytically detectable; b) a detection zone (DZ), which is located downstream of ASZ and exhibits an additional binding reactant (Capturer) firmly anchored to the matrix, and in which a complex (signal complex) containing the Capturer and the analyte and/or Reactant* is formed during the reaction, and ii) detecting the signal complex in the detection zone, the measured signal being used for determining the analyte. According to the invention, the flow matrix comprises at least one separation zone (SZ) between ASZ and DZ, which zone exhibits a structure (ligand) having binding capability for a component that is transported in the matrix and which would affect the measurable signal if the component is transported into DZ. The invention also relates to a test kit comprising the flow matrix.

Ligand binding assay and kit with a separation zone for disturbing analytes

Analytical method and apparatus

A system for administering certificates involves the generation, distribution and recall of certificates for public key systems. The generation comprises generating encryption keys and personalizing smart cards. The system is designed as a distributed system and is divided into a central unit with one or more associated terminal units. Each terminal and center is allocated unique certified identities. Mutual checking of access rights and data contents is carried out between the central unit and each terminal unit. An issued certificate can be traced back to the individual responsible for the issuing, compatibility with standards being seen to exist. Personalization of cards is integrated in each terminal.

Arrangement and method for a system for administering certificates

Enzyme activity determinations method characterized by the using of

Therapeutically active organic compound exhibiting at least one group comprising a structure --S'--S'--R, wherein the group --S'--R is defined by R being an organic group comprised in a physiologically acceptable compound H--S'--R obtainable by the splitting of the disulfide bridge --S'--S'-- in said structure, in which compound H--S'--R the sulfur atom S' is bound to a carbon atom in a heterocyclic aromatic ring in R having a configuration being such that the compound H--S'--R, while maintaining physiological acceptability, is stabilized, by tautomerism or resonance involving the sulfur atom S' bound to R, so as to be mainly excluded from reaction involving thiol disulfide exchange; S' being bound to an aliphatic carbon atom; and said compound, in addition to the group or groups comprising the structure --S'--S'--R, consisting of a residue of a therapeutically active organic base compound of non-polypeptide structure bound to S'. The invention also relates to a method of modifying a therapeutically active organic compound of non-polypeptide structure by introducing a structure --S'--S'--R according to above; a method of treating diseases by administration of a compound according to the invention and also to a pharmaceutical composition containing at least one compound according to the invention.

Therapeutically active compound and its use

Therapeutically active organic compound exhibiting at least one radical comprising a structure --S'--S'--R, where S'--R is defined by R being an organic group comprised in a physiologically acceptable compound H--S'--R obtainable by the splitting of the disulfide bridge --S'--S'-- in said structure, in which compound H--S'--R the sulfur atom S' is bound to a carbon atom in a heterocyclic aromatic ring in R having a configuration being such that the compound H--S'--R, while maintaining physiological acceptability, is stabilized, by tautomerism or resonance involving the sulfur atom S' bound to R, so as to be mainly excluded from further reaction involving thiol disulfide exchange; S' being bound to an aliphatic carbon atom; and said compound, in addition to the group or groups comprising the structure --S'--S'--R, also consisting of a residue of a therapeutically active organic base compound of polypeptide structure bound to S', for therapeutical use in mammals, including humans. The invention also relates to a pharmaceutical composition containing as an active substance the compound mentioned above.

Therapeutically active compound and pharmaceutical composition

Method for splitting di-sulphide bonds and a compound obtained thereby

Novel pyridine compounds having the formula R.sup.1 -S-S-A-Z are disclosed, in which formula R.sup.1 is 2-pyridyl, 5-nitro-2-pyridyl or 4-pyridyl, A is a hydrocarbon residue having 1-10 carbon atoms and Z is a group ##STR1## or acid addition salts of the last mentioned group, where n is 2 or 3, R.sup.1 has the same significance as R.sup.1 above and is equal thereto and R.sup.2 is methyl or ethyl. These compounds are particularly useful as bifunctional coupling agents and as thiolating agents.

Pyridine compounds modifying proteins, polypeptides or polysaccharides

An improvement in assaying methods involving biospecific affinity reactions, in which there are used from 2 to 4 reactants, one of which, reactant (I), is labelled with at least one analytically indicatable atom or group and is soluble in the aqueous liquid in which the biospecific affinity reaction is carried out, the reactants forming, by means of biospecific reactions, a conjugate in which labelled reactant (I) is incorporated; and in which assaying methods the analytically indicatable atom or group is assayed in the conjugate and/or in labelled reactant (I), which is not bound to the conjugate. The conjugate that has been formed or labelled reactant (I) not bound to the conjugate is bound covalently to an insoluble carrier or to an insolubilizable carrier, which latter carrier is made insoluble after the covalent binding has been carried out, whereafter the assay of the analytically indicatable atom or group is carried out.

Assaying methods involving biospecific affinity reactions

A reagent for use in immunochemical assay methods carried out in the presence of an aqueous liquid, which reagent comprises a conjugate of one or more molecules of immunoglobulin and one or more units of an analytically indicatable group which molecules and units are bound together via bridges containing the group, --S--S--, said conjugate being soluble in said aqueous liquid.

Reagent for use in immunochemical assay methods

A novel class of reactive derivatives of polymers containing HS-groups is disclosed in which derivatives a number of the HS-groups are converted to a disulphide group of the formula -S-S-A-Z, where A is a hydrocarbon residue having 1-10 carbon atoms and Z is a group ##STR1## or acid addition salts of the last mentioned group, where n is 2 or 3, R.sup.1 is 2-pyridyl, 5-nitro-2-pyridyl or 4-pyridyl and R.sup.2 is methyl or ethyl. The novel derivatives may be used, for example, as thiolating agents or as carriers for e.g. enzymes or other proteins etc.

Growing community of inventors

Upsala, Sweden

Jan P Carlsson