Danisco US Inc.

Genencor International, Inc.

Gist-brocades N.v.

Rijksuniversiteit Groningen

Academisch Ziekenhuis Groningen

Danisco US Inc., Genencor Division

Jan Maarten Van Dijl

Sierd Bron

Jan D H Jongbloed

Joerg Muller

Wilhelmus J Quax

Gerard Venema

Girbe Buist

Herman Groen

Hilde E Smith

Neeltje Margaretha Kooi

Harold Tjalsma

Roelof Hendrik Matthijs Kouwen

Jean-Yves Francois Dubois

Goffe Sytse Elsinga

Hendrik Petrus Jozef Bonarius

Jean-Yves François Dubois

The invention relates to the field of immunology and vaccine development. In particular, it relates to antibodies and fragments thereof againstand to therapeutic and diagnostic uses thereof. Provided is an isolated antibody or a functional fragment thereof, which binds to an epitope of the Immunodominant Staphylococcal antigen A (IsaA), wherein said antibody comprises at least four Isa A epitope-binding CDR sequences selected from the group consisting of SEQ ID NO: 1; SEQ ID NO: 2; SEQ ID NO: 3; SEQ ID NO: 4; SEQ ID NO: 5; SEQ ID NO: 6, and functional equivalents thereof having an amino acid sequence that is at least 85% identical to an amino acid sequence of SEQ ID NO: 1-6.

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The invention provides for genetically modifiedhost cells having decreased cytoplasmic reductase activity, such as decreased TrxA activity, and a nucleic acid encoding a heterologous Staphylococcal oxidase. The invention also provides for methods of producing di-sulfide bond containing proteins using the host cells of the invention and methods of improving protein folding using the host cell of the invention.

Host cells and methods of producing disulfide bond containing proteins

Described herein are methods to enhance protein secretion in a host cell. In preferred embodiment, the host cell is a gram-positive microorganism such as a. In another preferred embodiment, the host cell is a gram-negative microorganism. Preferably the gram-negative microorganism is anor a member of the genus. Protein secretion may be enhanced by the overexpression of protein components of the Tat pathway. Alternatively, secretion of foreign proteins can be selectively enhanced by forming a chimeric polypeptide comprising a tat signal sequence and the protein of interest. In a preferred embodiment, the tat signal sequence is selected from phoD or LipA.

The present invention provides methods and compositions for the efficient and enhanced secretion of a protein of interest from a host cell. In specific, proteins are secreted through the Sec-dependent pathway, involving the spoIIIJ and/or yqjG gene product(s). In some embodiments, expression of the spoIIIJ and/or yqjG gene product(s) is modulated by a promoter operably linked to the gene.

Oxa1p enhanced protein secretion

The subject invention describes the cloning and overexpression of leader peptidase genes. A method for isolating a leader peptidase gene is disclosed. Overexpression of the signal peptidase in a suitable host species leads to an enhanced rate of protein processing.

Processing of proteins

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Harkstede, Netherlands

Jan Maarten Van Dijl