Applied Nanosystems B.v.

Dsm IP Assets B.v.

Quest International B.v.

Unilever Patent Holdings B.v.

Van Den Bergh Foods Co., Division of Conopco, Inc.

Skw Biosystems

Friesland Brands B.v.

Jan Kok

Girbe Buist

Cornelis Johannes Leenhouts

Gerard Venema

Oscar Paul Kuipers

Ranjan Ramasamy

Anton Steen

Adrianus M Ledeboer

Herwig Bachmann

Marke Beerthuyzen

Thom Huppertz

Mariya Alexeevna Tarazanova

Jan Maat

Fabien Prevots

Josephus Mauritius Van Der Vossen

Marc Bigret

Gerhardus Venema

Blandine Fayard

Jan Sikkema

Arjan Nauta

Robèr Antoine Kemperman

Jan W Sanders

Arjen Nauta

Gerhard Hup

Christiaan Daniël Den Hengst

Olivera Gajic

Johannes Marie Wilhelmus Geurts

The present invention relates to the field of microbiology, specifically the field of production of a dairy food product using fermentation and the field of gene transfer by conjugation.

Method for the production of a dairy food product and a method for gene transfer by conjugation

Methods for improving binding of a proteinaceous substance to cell-wall material of a Gram-positive bacterium are disclosed. The proteinaceous substance includes an AcmA cell-wall binding domain, homolog or functional derivative thereof. The method includes treating the cell-wall material with a solution capable of removing a cell-wall component such as a protein, lipoteichoic acid or carbohydrate from the cell-wall material and contacting the proteinaceous substance with the cell-wall material.

Immunization with bacterial ghost-based vaccines

The invention relates to surface display of proteins on microorganisms via the targeting and anchoring of heterologous proteins to the outer surface of cells such as yeast, fungi, mammalian, plant cells, and bacteria. The invention provides a proteinaceous substance comprising a reactive group and at least one attaching peptide including a stretch of amino acids having a sequence corresponding to at least a part of the consensus amino acid sequence listed in FIG.and further includes a method for attaching a proteinaceous substance to the cell wall of a microorganism comprising the use of the attaching peptide.

Attaching substances to microorganisms

The invention relates to surface display of proteins on micro-organisms via the targeting and anchoring of heterologous proteins to the outer surface of cells such as yeast, fungi, mammalian and plant cells, and bacteria. The invention provides a proteinaceous substance comprising a reactive group and at least one attaching peptide which comprises a stretch of amino acids having a sequence corresponding to at least a part of the consensus amino acid sequence listed in FIG.and comprises a method for attaching a proteinaceous substance to the cell wall of a micro-organism comprising the use of said attaching peptide.

Attaching substances to micro-organisms

Method to provide bacterial ghosts provided with antigens

Bacterial ghosts provided with antigens

Complex inducible promoter system derivable from a phage of a lactic

Salt-inducible promoter derivable from a lactic acid bacterium, and its

Process for the lysis of a culture of lactic acid bacteria by means of a

Use of a Sec-dependent secretion system for secreting proteins that are

The claimed invention is drawn to a recombinant plasmid which can replicate in Bacillus subtilis, Escherichia coli, and lactic acid Streptococcus bacteria comprising the replication of origin from Streptococcus cremoris plasmid pWV01 as its origin of replication, in addition to coding marker genes and genes of interest which code for improved fermenting properties.

Plasmids replicatable in Bacillus subtilis, E. coli and lactic acid

Growing community of inventors

Groningen, Netherlands

Jan Kok