Lever Brothers Company, Division of Conopco, Inc.

Lever Brothers Company

Novo Nordisk A/s

Other

Jan Klugkist

David Thom

Claus Von Der Osten

Peter Bauditz

Willem R Van Dijk

Peter Markvardsen

Cornelis A Lagerwaard

Johannes M Cornelissen

Laurens Nicolaas Sierkstra

Ton Swarthoff

Eric Casteleijn

Pieter Dirk Van Wassenaar

Swarthoff Ton

The present invention relates to enzymes produced by mutating the genes for a number of subtilases and expressing the mutated genes in suitable hosts are presented. The enzymes exhibit improved stability and/or improved wash performance in any detergent in comparison to their wild type parent enzymes. The enzymes are well-suited for use in any detergent and for some in especially liquid or solid shaped detergent compositions.

Protease variants and compositions

Subtilase variants

There is provided an enzymatic detergent composition comprising one or more surfactants and an endoglucanase which is not a Family 7 cellulase, which contains no cellulose binding domain and wherein the catalytic domain contains at least two disulphide bridges. Preferably, the endoglucanase is producible from Thermomonospora fusca, or mutants or variants thereof.

Enzymatic detergent compositions

There is provided an enzymatic detergent composition comprising a surfactant and an endoglucanase producible from Thermomonospora fusca, preferably E5, or mutants or variants thereof.

A detergent composition comprising an anionic surfactant, a nonionic surfactant and a lipase enzyme, characterised in that:

Enzymatic detergent composition

A dishwashing or rinsing composition comprising a surfactant and a chlorine-type bleaching agent, characterised in that it further comprises a lipolytic enzyme in an amount in the range 0.005 to 100 lipase units per mg (dry wt.) of the composition.

Enzymatic dishwashing composition containing a chlorine-type bleaching

An enzymatic detergent and bleaching composition contains a bleaching system which is stronger than the sodium perborate/TAED system. In such a composition a lipase is employed which is produced by cloning, by rDNA technique, the gene which encodes the lipase produced by the fungas Humicola lanuginosa and expressing that gene in Aspergillus oryzae as host. The lipase is used in an amount giving a lipolytic enzyme activity of from 100 to 0.005 lipolytic units/mg of the composition.

Enzymatic detergent and bleaching composition containing a specific rDNA

The invention relates to the use of a certain class of lipases together with strong bleaching agents in detergent compositions. This class of lipases consists of fungal lipases ex Humicola lanuginosa or Thermomyces lanuginosus, and bacterial lipases which show a positive immunological cross-reaction with the antibody of the lipase produced by Chromobacter viscosum var. lipolyticum NRRL B-3673. The strong bleaching agents are stronger than the sodium perborate/TAED system, i.e. stronger than peracetic acid or they yield, on perhydrolysis, a peracid faster than the sodium perborate/TAED system.

Growing community of inventors

Vlaardingen, Netherlands

Jan Klugkist