Amersham Biosciences Ab

Ge Healthcare Bio-sciences Ab

Jan Gustavsson

Jean-Luc Maloisel

Bo-Lennart Johansson

Makonnen Belew

Mikael Andersson

A method for the removal of a substance carrying a negative charge and being present in an aqueous liquid (I). The method comprises the steps of: (i) contacting the liquid with a matrix carrying a plurality of ligands comprising a positively charged structure and a hydrophobic structure, and (ii) desorbing the substance. The characterizing feature is that (I) each of said ligands together with a spacer has the formula: &mdash;SP&mdash;&lsqb;Ar&mdash;R &mdash;N (R R R )&rsqb; where (A) &lsqb;Ar&mdash;R &mdash;N (R R R )&rsqb; represents a ligand a) Ar is an aromatic ring, b) R is &lsqb;(L) R&prime; &rsqb; where n and m are integers selected amongst zero or 1; L is amino nitrogen, ether oxygen or thioether sulphur; R&prime; is a linker selected among 1) hydrocarbon groups; 2) &mdash;C(&boxH;NH)&mdash;; c) R are selected among hydrogen and alkyls; (B) SP is a spacer providing a carbon or a heteroatom directly attached to Ar&mdash;R &mdash;N (R R R ); (C)&mdash;represents that SP replaces a hydrogen in (Ar&mdash;R &mdash;N (R R R ); (D)&mdash;represents binding to the matrix; and (II) desorption. There is also described (a) anion-exchangers having high breakthrough capacities, (b) a screening method and (c) a desalting protocol.

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Uppsala, Sweden

Jan Gustavsson