Pharmacia Ab

Pharmacia Biotech Ab

Amersham Pharmacia Biotech Ab

Amersham Pharmacia Biotech Aktiebolag

Amersham Biociences Ab

Jan-Gunnar Gustafsson

Mats Carlsson

Per Hedman

Annika Lindgren

Per-&angst;ke Pernemalm

J&ouml;rgen L&ouml;nngren

Jorgen Lonngren

Johan &Ouml;hman

Per-Ake Pernemalm

A method for recovering a desired component from a sample solution with beads under stabilized fluidized bed conditions is provided. Also provided are support beads for use in downstream processing which comprise a polymer matrix into which glass or silica particles have been incorporated.

Process and means for down stream processing

Methods for producing correctly folded biological active recombinant protein or polypeptide comprise the steps of expressing the protein or polypeptide in prokaryotic cells, harvesting the cells, directly solubilizing the cells in a buffer at pH of about 8 to 11 with the chaotropic agent and a reducing agent, and diluting the solution with water and a diluent. The method is conducted in the absence of mechanical disruption of the cells and isolation and washing of refractile bodies.

Method for producing a correctly folded, biological active recombinant protein

Support beads for downstream processing including a polymer, esp. a polysaccharide, matrix into which glass or silica particles, preferably quartz particles, have been incorporated, and their use in downstream processing, especially stabilized fluidized bed separations.

Support beads for downstream processing comprising a polymer, esp. a polysaccharide, matrix into which glass or silica particles, preferably quartz particles, have been incorporated, and their use in downstream processing, especially stabilized fluidized bed separations.

Growing community of inventors

Uppsala, Sweden

Jan-Gunnar Gustafsson