Cargill Inc.

Puratos Nv

K.u. Leuven Research & Development

Other

University of Connecticut

Katholieke Universiteit Leuven

Danisco A/s

Fugeia Nv

Theo Coppens

Jan Delcour

Dirk Iserentant

Willem F Broekaert

Christophe Courtin

Filip Arnaut

Jacques Georis

Fabienne Verte

Valérie Dorgeo

Ellen Fierens

Kristof Brijs

Wim Veraverbeke

Geertrui Bosmans

Winok Debyser

Richard Steven Parnas

Rita M Delrue

Ignace H Verpoest

Ingrid Van Haesendonck

Kurt Gebruers

Hans Goesaert

Katleen Fierens

Johan Robben

Steven Van Campenhout

Chris Michiels

Iris Noots

Bram Damen

Dara Woerdeman

Valerie Dorgeo

Ingrid Paula Van Haesendonck

Willem Frans Broekaert

Ingrid Paula Hilde Van Haesendonck

The present invention concerns a method for selectively activating a thermostable hydrolase at a temperature above T1. The present invention provides compositions comprising a thermostable hydrolase and a temperature sensitive inhibitor, wherein said thermostable hydrolase and said temperature sensitive inhibitor form a hydrolase-inhibitor complex at a temperature below T1, but which dissociates at a temperature of about T1. The present invention also relates to uses of said compositions, and a method for preparing said compositions.

Enzyme-inhibitor complexes comprising a thermostable hydrolase and a temperature sensitive inhibitor

The present invention concerns a method for selectively activating a thermostable hydrolase at a temperature above T. The present invention provides compositions comprising a thermostable hydrolase and a temperature sensitive inhibitor, wherein said thermostable hydrolase and said temperature sensitive inhibitor form a hydrolase-inhibitor complex at a temperature below T, but which dissociates at a temperature of about T. The present invention also relates to uses of said compositions, and a method for preparing said compositions.

Methods for selectively activating a thermostable hydrolase

The present invention provides a method for the extraction and isolation of soluble arabinoxylan products from cereal grain. Preferably, such soluble arabinoxylan product is any one of soluble arabinoxylan, arabinoxylan-oligosaccharides, xylose, arabinose, ferulic acid and mixtures thereof. Said method comprises partial debranning of whole cereal grains to obtain partially debranned cereal grains followed by roller milling of said partially debranned cereal grains to obtain cereal bran. The method further comprises the mashing of at least part of said cereal bran in water optionally involving the treatment of the mash with any one of an enzyme preparation, an acid, a base, a peroxide or combinations thereof, either simultaneously or sequentially, to solubilize and optionally depolymerize a fraction of the arabinoxylan comprised in said cereal bran. Preferably, said treatment is done with an enzyme preparation containing an endoxylanase. The method further comprises the separation from said mash of a solubilized fraction, which comprises at least part of the solubilized soluble arabinoxylan products.

Method for making pentoses and pentose-based soluble oligo/polysaccharides from cereal grain involving debranning technology

A method for increasing in a baked product, after baking, the level of water-soluble arabinoxylan with an average degree of polymerization ranging from 5 to 50, comprising preparing a dough for a baked product with flour having a total arabinoxylan content of at least 2.0%, and adding to the dough an enzyme preparation comprising at least one thermophilic endoxylanase in an amount at least two-fold higher than the amount conferring 90% of the maximal bread volume increase obtained by addition of such enzyme preparation to the recipe of the baked product. Using this method, baked products can be obtained that have a level of arabinoxylan of at least 1.7% The present invention further relates to baked products obtainable by this method, and to an improver and a dough which may be used for the preparation of such products.

Bread with increased arabinoxylo-oligosaccharide content

The present invention relates to the use of oligosaccharides derived from arabinoxylan for use in the prevention and treatment of gastrointestinal infection. More particularly the invention provides a method for preventing or reducing the gastrointestinal infection of a animal or human being with bacteria associated with gastroenteritis through the supplementation of their diets with the said oligosaccharides.

Arabinoxylo-oligosaccharides useful against gastrointestinal infections

The present invention relates to preparations comprising (arabino)xylan oligosaccharides with average degree of polymerisation between 4 and 10 and average degree of arabinose substitution between 0.15 and 0.35, which beneficially modulate the intestinal flora, such preparation comprising both xylo- and arabinoxylo-oligosaccharides. Preferably, the arabinoxylooligosaccharides are substituted in part by hydroxycinnamic acids and exert antioxidant effects. Furthermore, it is preferred that the preparations according to the present invention have a nitrogen content below 0.16% (w/w) in order to ensure good organoleptic and color properties, particularly upon heating said preparations. In addition, food, beverage and nutritional supplement products comprising the preparations are provided as well as methods to prepare the said preparations.

(Arabino)xylan oligosaccharide preparation

The present invention provides nutritional compositions, more particularly food supplements and processed food products, enriched with arabinoxylan-oligosaccharides and further comprising either or both water-unextractable arabinoxylans or water-soluble arabinoxylans. Preferably said nutritional compositions comprises both arabinoxylan-oligosaccharides and water-unextractable arabinoxylans.

Nutriment containing arabinoxylan and oligosaccharides

The present invention relates to compositions of low molecular mass arabinoxylans for use as dietary supplements and to methods of improving growth performance and feed utilization of animals through the supplementation of their diets with the said compositions. In a preferred embodiment, the low molecular mass arabinoxylans are derived from natural sources, such as plant material and more preferably of cereals. They can be selected fractions of said natural arabinoxylans or can be obtained by acid and/or enzymatic depolymerization or fragmentation of said natural arabinoxylans or they can be structural analogues produced by chemical and/or physical processes.

Non-starch-polysaccharides

The present invention provides a method for producing preparations comprising soluble arabinoxylans as co-products of ethanol production through fermentation of whole-grain cereals. The method results in preparations that are highly enriched in soluble arabinoxylans, which can be used as a food, beverage, or feed ingredients.

Method for making soluble arabinoxylans as co-product of fermentation of whole-grain cereals

This invention consists of a modified gluten biopolymer for use in industrial applications, such as composites and foams. In the present work, the fracture toughness of the gluten polymer was improved with the addition of a thiol-containing modifying agent. This work also resulted in the development of a gluten biopolymer-modified fiber bundle, demonstrating the potential to process fully biodegradable composite materials. Qualitative analysis suggests that a reasonably strong interface between the natural fibers and biopolymer matrix can form spontaneously under the proper conditions. Therefore this invention relates to a modified gluten biopolymer for use in industrial applications, such as composites, stabilized foams and molded articles of manufactures. The present invention relates to a new gluten based biopolymer with modified properties, such as an increase in impact strength, and prepared by using thiol-containing molecules. The multifunctional activity of the polythiol-containing molecules generates the potential for the development of a new material base for commodity plastics. The invention furthermore relates to a new composite material comprising gluten-coated fiber, its use and the method for preparing the composite material.

Gluten biopolymers

The present invention concerns an inhibitor of xylanolytic and/or &#x3b2;-glucanolytic enzymes, method for obtaining the inhibitor, said inhibitor and processes for obtaining micro-organism, plant or plant material wherein the activity of the inhibitor according to the invention is increased or reduced and to the use of the inhibitor, the cited micro-organism, plant or plant material in a variety of processes and applications.

Inhibitors of cellulolytic, xylanolytic and &#x3b2;-glucanolytic enzymes

Process for the preparation of malted cereals, wherein the steeping step includes one or more wetting stages at a temperature between 5 and 30&#xb0; C. preferably between 10 and 20&#xb0; C., until the material has a moisture content between 20 and 60% by weight, preferably between 38 and 47%, wherein after a germination period between 2 and 7 days, preferably between 3 to 6 days at a temperature between 10 and 30&#xb0; C., preferably between 14 and 18&#xb0; C., the steeped and germinated cereals are preferably kilned by increasing the temperature to values between 40 and 150&#xb0; C. until the material has a moisture content between 2 and 15% by weight, and wherein one or more microbial cultures selected from the group consisting of one or more bacteria and/or one of more activated spores are added in one or more times during the process.

Process for the preparation of malted cereals

The present invention concerns a method for the separation and/or isolation of inhibitors of cellulolytic, xylanolytic and/or beta-glucanolytic enzymes, inhibitors obtainable by said method, and process for obtaining micro-organism, plant or plant material wherein the activity of the inhibitor according to the invention is increased or reduced and to the use of the inhibitor, using the cited micro-organism, plant or plant material and/or the use of endoxylanases selected or modified using these inhibitors in a variety of process and applications.

Biocatalyst inhibitors

The invention provides process or method of malting cereal seeds, such as barley, wheat, rye, corn, oats, rice, millet and sorghum which process includes reducing the microbial level in the seeds prior to steeping, malting and the introduction of a starter culture used in the malting process. According to the invention, the reduction of microbial levels permits efficient use of starter cultures, such as molds, yeasts, bacteria, spores and activated spores.

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Heverlee, Belgium

Jan Delcour