Danisco US Inc.

Genencor International, Inc.

Jan D H Jongbloed

Jan Maarten Van Dijl

Sierd Bron

Joerg Muller

Described herein are methods to enhance protein secretion in a host cell. In preferred embodiment, the host cell is a gram-positive microorganism such as a. In another preferred embodiment, the host cell is a gram-negative microorganism. Preferably the gram-negative microorganism is anor a member of the genus. Protein secretion may be enhanced by the overexpression of protein components of the Tat pathway. Alternatively, secretion of foreign proteins can be selectively enhanced by forming a chimeric polypeptide comprising a tat signal sequence and the protein of interest. In a preferred embodiment, the tat signal sequence is selected from phoD or LipA.

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Groningen, Netherlands

Jan D H Jongbloed