Bestewil Holding B.v.

Rijksuniversiteit Groningen

Jan Christiaan Wilschut

Antonius Johannes Hendrikus Stegmann

Anke Luise Wilhelmine Huckriede

Jørgen Martin De Jonge

Pieter Joseph Schoen

Johannes Henricus Gerardus Van Berkum

Djoeke Geesje Regts

Marijke Holtrop

Catharina Arnoldine H H Daemen

The invention relates to the production of virosome-like-particles. The invention provides a method for producing a virosome-like-particle comprising contacting an enveloped virus with a solution containing a short-chain phospholipid allowing solubilisation of the viral envelope of said virus further comprising removing short-chain phospholipid from said solution allowing formation of a functionally reconstituted viral envelope.

Virosome-like-particles

Vaccines directed against antigens such as membrane proteins from pathogens or tumor cells are disclosed. Also described are methods of forming reconstituted viral membranes, with membrane fusion activity, which are lipid bilayer membranes preferably containing natural lipids of a virus, a viral fusion protein, one or more optional further antigens as well as amphiphilic adjuvants. Pharmaceutical compositions including such reconstituted viral membranes are also described.

Functionally reconstituted viral membranes containing adjuvant

An alphavirus vector system comprising nucleic acid of a human papilloma virus origin is disclosed. A method of treating or preventing cervical cancer is also disclosed. The method includes administering the alphavirus vector system and/or a cell comprising nucleic acid derived from human papilloma virus (HPV) to a subject. The alphavirus vector system or the cell may be administered as a vaccine.

Genetic immunization against cervical carcinoma

Growing community of inventors

Garnwerd, Netherlands

Jan Christiaan Wilschut