Masarykova Univerzita

Enantis S.r.o.

Jan Brezovsky

Jiri Damborsky

Kamil Paruch

Zbynek Prokop

Stepan Havel

Lumir Krejci

Benoit Carbain

Fedor Nikulenkov

Lukas Daniel

Alexandra Sisakova

Veronika Stepankova

Radka Chaloupkova

Pavel Krejci

Lukas Balek

Artur Wiktor Gora

Michaela Bosakova

Eva Sebestova

Pavel Vanacek

Livia Eiselleova

David Bednar

Pavel Dvorak

Zaneta Konecna

Petr Dvorak

Eva Chovancova

Tana Koudelakova

The invention provides an isolated thermostable polypeptide possessing FGF2 activity and having at least 85% sequence identity to SEQ ID NO: 2 (FGF2 wt) or a functional fragment thereof, and comprising at least one amino acid substitution R31L and the use thereof in the cell biology research, regenerative medicine and related medical applications or cosmetics. Further it discloses a culture medium comprising subjected FGF2 suitable for culturing a human pluripotent stem cells involving both human embryonic stem cells and induced pluripotent stem cells.

Thermostable FGF2 polypeptide, use thereof

Compounds represented by the structural formula (1) R1, R2, R3, R4, R5, R6 are inhibitors of nucleases, and are useful in particular in a method of treatment and/or prevention of proliferative diseases, neurodegenerative diseases, and other genomic instability associated diseases.

Substituted aminothiazoles as inhibitors of nucleases

The invention provides compounds represented by the structural formula (1): wherein R, R, Rand Rare as defined in the claims. The compounds are inhibitors of nucleases, and are useful in particular in a method of treatment and/or prevention of proliferative diseases, neurodegenerative diseases, and other genomic instability associated diseases.

Pyrazolotriazines as inhibitors of nucleases

Thermostabilization of a protein where the protein contains access routes and wherein at least one amino acid in the bottleneck of the access route is mutated, includes identifying the amino acids of the bottleneck and the amino acids control exchange of the solvent between a buried protein core and surrounding environment and/or in the packing of the amino acids inside the access route. Modification of the amino acids are determined so that the packing of the amino acids inside the tunnel is improved and the access route prevents access of undesired solvent molecules to the protein core, while allowing passage of the compounds necessary at the protein core to enable the protein to perform its biological function.

Method of thermostabilization of a protein and/or stabilization towards organic solvents

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Brno, Czechia

Jan Brezovsky