Astra Aktiebolag

Aktiebolaget Astra

Jan Birger Tornell

Stig Lennart Hansson

Lennart Gustav Lundberg

Michael Edlund

Lars Gustav Blackberg

Olle Carl Hernell

Mats Olof Stromqvist

Jeanette A Nilsson

Ulf F Lidberg

Curt S Enerback

Peter N Carlsson

Karl G Bjursell

The invention discloses nucleic acids encoding variant Bile Salt Stimulated Lipase (BSSL; EC 3.1.1.1). The encoded variant BSSL enzymes maintain catalytic activity but contain fewer glycosylation sites that full-length BSSL. This reduced glycosylation facilitates purification and characterization of recombinant BSSL proteins.

Nucleic acids encoding BSSL variants

The present invention relates to novel polypeptides which are variants of Bile Salt-Stimulated Lipase (BSSL; EC 3.1.1.1). It also relates to DNA molecules encoding the said polypeptides, and to subproducts comprising the said DNA molecules. The invention further relates to processes for producing the said BSSL variants and for producing transgenic non-human mammals capable of expressing the BSSL variants. Furthermore the invention relates to such transgenic animals as well as to infant formulas comprising milk from such transgenic animals. The invention also relates to pharmaceutical compositions comprising the said polypeptides; and the use of the said polypeptides and DNA molecules for the manufacture of medicaments.

Transgenic non-human mammals producing BSSL variants

The present invention relates to a DNA molecule containing intron sequences and encoding a human protein which is, depending on the site of action, called Bile Salt-Stimulated Lipase (BSSL) or Carboxyl Ester Lipase (CEL). The DNA molecule is advantageously used in the production of recombinant human BSSL/CEL, preferably by means of production in transgenic non-human mammals. The recombinant human BSSL/CEL can be used as a constituent of infant formulas used for feeding infants as a substitute for human milk, or in the manufacture of medicaments against e.g. fat malabsorption, cystic fibrosis and chronic pancreatitis.

Transgenic non-human mammals that express human BSSL/CEL

Genomic DNA sequences encoding human BSSL/CEL

Growing community of inventors

Vastra Frolunda, Sweden

Jan Birger Tornell